Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS20175

Experiment: 36hr PHYTO2 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS20170 and BPHYT_RS20175 are separated by 56 nucleotidesBPHYT_RS20175 and BPHYT_RS20180 overlap by 4 nucleotides BPHYT_RS20170: BPHYT_RS20170 - sugar transporter, at 68,137 to 70,359 _RS20170 BPHYT_RS20175: BPHYT_RS20175 - protein-tyrosine-phosphatase, at 70,416 to 70,868 _RS20175 BPHYT_RS20180: BPHYT_RS20180 - sugar ABC transporter substrate-binding protein, at 70,865 to 71,917 _RS20180 Position (kb) 70 71Strain fitness (log2 ratio) -2 -1 0 1 2at 69.871 kb on - strand, within BPHYT_RS20170at 70.860 kb on - strandat 70.889 kb on + strandat 71.069 kb on + strand, within BPHYT_RS20180at 71.182 kb on + strand, within BPHYT_RS20180at 71.251 kb on + strand, within BPHYT_RS20180

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Per-strain Table

Position Strand Gene LocusTag Fraction 36hr PHYTO2 MOI 1
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69,871 - BPHYT_RS20170 0.78 -1.8
70,860 - +0.6
70,889 + +2.7
71,069 + BPHYT_RS20180 0.19 +0.8
71,182 + BPHYT_RS20180 0.30 +2.1
71,251 + BPHYT_RS20180 0.37 -1.5

Or see this region's nucleotide sequence