Experiment: 36hr PHYTO2 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS18630 and BPHYT_RS18635 are separated by 103 nucleotides BPHYT_RS18635 and BPHYT_RS18640 are separated by 264 nucleotides
BPHYT_RS18630: BPHYT_RS18630 - allophanate hydrolase, at 4,189,732 to 4,190,784
_RS18630
BPHYT_RS18635: BPHYT_RS18635 - LamB/YcsF family protein, at 4,190,888 to 4,191,640
_RS18635
BPHYT_RS18640: BPHYT_RS18640 - membrane protein, at 4,191,905 to 4,192,654
_RS18640
Position (kb)
4190
4191
4192 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4190.066 kb on - strand, within BPHYT_RS18630 at 4190.475 kb on + strand, within BPHYT_RS18630 at 4190.703 kb on + strand at 4190.704 kb on - strand at 4190.704 kb on - strand at 4190.707 kb on + strand at 4190.708 kb on - strand at 4190.708 kb on - strand at 4190.782 kb on + strand at 4190.783 kb on - strand at 4191.122 kb on + strand, within BPHYT_RS18635 at 4191.137 kb on + strand, within BPHYT_RS18635 at 4191.138 kb on - strand, within BPHYT_RS18635 at 4191.774 kb on - strand at 4191.774 kb on - strand at 4191.774 kb on - strand at 4191.774 kb on - strand at 4191.785 kb on + strand at 4191.925 kb on - strand at 4191.925 kb on - strand at 4191.925 kb on - strand at 4191.969 kb on + strand at 4191.969 kb on + strand at 4192.088 kb on - strand, within BPHYT_RS18640 at 4192.214 kb on + strand, within BPHYT_RS18640 at 4192.215 kb on - strand, within BPHYT_RS18640 at 4192.215 kb on - strand, within BPHYT_RS18640 at 4192.215 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO2 MOI 1 remove 4,190,066 - BPHYT_RS18630 0.32 -1.2 4,190,475 + BPHYT_RS18630 0.71 -1.3 4,190,703 + +1.0 4,190,704 - +1.2 4,190,704 - +0.7 4,190,707 + -2.3 4,190,708 - -0.2 4,190,708 - +0.4 4,190,782 + -1.0 4,190,783 - +0.1 4,191,122 + BPHYT_RS18635 0.31 +2.0 4,191,137 + BPHYT_RS18635 0.33 +1.6 4,191,138 - BPHYT_RS18635 0.33 +1.0 4,191,774 - +0.2 4,191,774 - +0.7 4,191,774 - +0.2 4,191,774 - -0.5 4,191,785 + -0.0 4,191,925 - +1.5 4,191,925 - +0.9 4,191,925 - -0.6 4,191,969 + -0.5 4,191,969 + -0.1 4,192,088 - BPHYT_RS18640 0.24 -0.1 4,192,214 + BPHYT_RS18640 0.41 +1.4 4,192,215 - BPHYT_RS18640 0.41 -0.7 4,192,215 - BPHYT_RS18640 0.41 -1.0 4,192,215 - +0.6
Or see this region's nucleotide sequence