Experiment: 36hr PHYTO2 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS18620 and BPHYT_RS18625 are separated by 205 nucleotides BPHYT_RS18625 and BPHYT_RS18630 overlap by 4 nucleotides BPHYT_RS18630 and BPHYT_RS18635 are separated by 103 nucleotides
BPHYT_RS18620: BPHYT_RS18620 - transcriptional regulator, at 4,188,364 to 4,188,876
_RS18620
BPHYT_RS18625: BPHYT_RS18625 - allophanate hydrolase, at 4,189,082 to 4,189,735
_RS18625
BPHYT_RS18630: BPHYT_RS18630 - allophanate hydrolase, at 4,189,732 to 4,190,784
_RS18630
BPHYT_RS18635: BPHYT_RS18635 - LamB/YcsF family protein, at 4,190,888 to 4,191,640
_RS18635
Position (kb)
4189
4190
4191 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4188.921 kb on - strand at 4188.990 kb on - strand at 4188.995 kb on - strand at 4189.076 kb on + strand at 4189.093 kb on + strand at 4189.093 kb on + strand at 4189.094 kb on - strand at 4189.094 kb on - strand at 4189.122 kb on + strand at 4189.123 kb on - strand at 4189.454 kb on + strand, within BPHYT_RS18625 at 4189.455 kb on - strand, within BPHYT_RS18625 at 4189.455 kb on - strand, within BPHYT_RS18625 at 4189.493 kb on + strand, within BPHYT_RS18625 at 4189.493 kb on + strand, within BPHYT_RS18625 at 4189.494 kb on - strand, within BPHYT_RS18625 at 4189.523 kb on - strand, within BPHYT_RS18625 at 4189.523 kb on - strand, within BPHYT_RS18625 at 4189.726 kb on - strand at 4190.066 kb on - strand, within BPHYT_RS18630 at 4190.475 kb on + strand, within BPHYT_RS18630 at 4190.703 kb on + strand at 4190.704 kb on - strand at 4190.704 kb on - strand at 4190.707 kb on + strand at 4190.708 kb on - strand at 4190.708 kb on - strand at 4190.782 kb on + strand at 4190.783 kb on - strand at 4191.122 kb on + strand, within BPHYT_RS18635 at 4191.137 kb on + strand, within BPHYT_RS18635 at 4191.138 kb on - strand, within BPHYT_RS18635 at 4191.774 kb on - strand at 4191.774 kb on - strand at 4191.774 kb on - strand at 4191.774 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO2 MOI 1 remove 4,188,921 - -0.4 4,188,990 - -0.4 4,188,995 - -1.6 4,189,076 + +0.4 4,189,093 + -0.3 4,189,093 + -0.9 4,189,094 - -0.0 4,189,094 - -1.6 4,189,122 + +1.3 4,189,123 - -1.5 4,189,454 + BPHYT_RS18625 0.57 -2.3 4,189,455 - BPHYT_RS18625 0.57 +0.2 4,189,455 - BPHYT_RS18625 0.57 -0.9 4,189,493 + BPHYT_RS18625 0.63 -1.5 4,189,493 + BPHYT_RS18625 0.63 +0.3 4,189,494 - BPHYT_RS18625 0.63 -1.5 4,189,523 - BPHYT_RS18625 0.67 +1.6 4,189,523 - BPHYT_RS18625 0.67 +0.5 4,189,726 - -0.3 4,190,066 - BPHYT_RS18630 0.32 -1.2 4,190,475 + BPHYT_RS18630 0.71 -1.3 4,190,703 + +1.0 4,190,704 - +1.2 4,190,704 - +0.7 4,190,707 + -2.3 4,190,708 - -0.2 4,190,708 - +0.4 4,190,782 + -1.0 4,190,783 - +0.1 4,191,122 + BPHYT_RS18635 0.31 +2.0 4,191,137 + BPHYT_RS18635 0.33 +1.6 4,191,138 - BPHYT_RS18635 0.33 +1.0 4,191,774 - +0.2 4,191,774 - +0.7 4,191,774 - +0.2 4,191,774 - -0.5
Or see this region's nucleotide sequence