Experiment: 36hr PHYTO2 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS18615 and BPHYT_RS18620 are separated by 43 nucleotides BPHYT_RS18620 and BPHYT_RS18625 are separated by 205 nucleotides BPHYT_RS18625 and BPHYT_RS18630 overlap by 4 nucleotides
BPHYT_RS18615: BPHYT_RS18615 - 5-formyltetrahydrofolate cyclo-ligase, at 4,187,778 to 4,188,320
_RS18615
BPHYT_RS18620: BPHYT_RS18620 - transcriptional regulator, at 4,188,364 to 4,188,876
_RS18620
BPHYT_RS18625: BPHYT_RS18625 - allophanate hydrolase, at 4,189,082 to 4,189,735
_RS18625
BPHYT_RS18630: BPHYT_RS18630 - allophanate hydrolase, at 4,189,732 to 4,190,784
_RS18630
Position (kb)
4189
4190 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4188.156 kb on + strand, within BPHYT_RS18615 at 4188.159 kb on + strand, within BPHYT_RS18615 at 4188.220 kb on - strand, within BPHYT_RS18615 at 4188.220 kb on - strand, within BPHYT_RS18615 at 4188.248 kb on - strand, within BPHYT_RS18615 at 4188.330 kb on - strand at 4188.365 kb on + strand at 4188.367 kb on + strand at 4188.368 kb on - strand at 4188.578 kb on - strand, within BPHYT_RS18620 at 4188.578 kb on - strand, within BPHYT_RS18620 at 4188.582 kb on - strand, within BPHYT_RS18620 at 4188.588 kb on + strand, within BPHYT_RS18620 at 4188.592 kb on + strand, within BPHYT_RS18620 at 4188.612 kb on - strand, within BPHYT_RS18620 at 4188.723 kb on + strand, within BPHYT_RS18620 at 4188.724 kb on - strand, within BPHYT_RS18620 at 4188.921 kb on - strand at 4188.990 kb on - strand at 4188.995 kb on - strand at 4189.076 kb on + strand at 4189.093 kb on + strand at 4189.093 kb on + strand at 4189.094 kb on - strand at 4189.094 kb on - strand at 4189.122 kb on + strand at 4189.123 kb on - strand at 4189.454 kb on + strand, within BPHYT_RS18625 at 4189.455 kb on - strand, within BPHYT_RS18625 at 4189.455 kb on - strand, within BPHYT_RS18625 at 4189.493 kb on + strand, within BPHYT_RS18625 at 4189.493 kb on + strand, within BPHYT_RS18625 at 4189.494 kb on - strand, within BPHYT_RS18625 at 4189.523 kb on - strand, within BPHYT_RS18625 at 4189.523 kb on - strand, within BPHYT_RS18625 at 4189.726 kb on - strand at 4190.066 kb on - strand, within BPHYT_RS18630 at 4190.475 kb on + strand, within BPHYT_RS18630 at 4190.703 kb on + strand at 4190.704 kb on - strand at 4190.704 kb on - strand at 4190.707 kb on + strand at 4190.708 kb on - strand at 4190.708 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO2 MOI 1 remove 4,188,156 + BPHYT_RS18615 0.70 -0.4 4,188,159 + BPHYT_RS18615 0.70 -2.5 4,188,220 - BPHYT_RS18615 0.81 -0.2 4,188,220 - BPHYT_RS18615 0.81 +0.2 4,188,248 - BPHYT_RS18615 0.87 -0.6 4,188,330 - +1.0 4,188,365 + -0.9 4,188,367 + -1.8 4,188,368 - -1.1 4,188,578 - BPHYT_RS18620 0.42 -0.2 4,188,578 - BPHYT_RS18620 0.42 +1.6 4,188,582 - BPHYT_RS18620 0.42 +0.0 4,188,588 + BPHYT_RS18620 0.44 +1.0 4,188,592 + BPHYT_RS18620 0.44 +1.0 4,188,612 - BPHYT_RS18620 0.48 +0.6 4,188,723 + BPHYT_RS18620 0.70 +2.6 4,188,724 - BPHYT_RS18620 0.70 +0.6 4,188,921 - -0.4 4,188,990 - -0.4 4,188,995 - -1.6 4,189,076 + +0.4 4,189,093 + -0.3 4,189,093 + -0.9 4,189,094 - -0.0 4,189,094 - -1.6 4,189,122 + +1.3 4,189,123 - -1.5 4,189,454 + BPHYT_RS18625 0.57 -2.3 4,189,455 - BPHYT_RS18625 0.57 +0.2 4,189,455 - BPHYT_RS18625 0.57 -0.9 4,189,493 + BPHYT_RS18625 0.63 -1.5 4,189,493 + BPHYT_RS18625 0.63 +0.3 4,189,494 - BPHYT_RS18625 0.63 -1.5 4,189,523 - BPHYT_RS18625 0.67 +1.6 4,189,523 - BPHYT_RS18625 0.67 +0.5 4,189,726 - -0.3 4,190,066 - BPHYT_RS18630 0.32 -1.2 4,190,475 + BPHYT_RS18630 0.71 -1.3 4,190,703 + +1.0 4,190,704 - +1.2 4,190,704 - +0.7 4,190,707 + -2.3 4,190,708 - -0.2 4,190,708 - +0.4
Or see this region's nucleotide sequence