Experiment: 36hr PHYTO2 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS18595 and BPHYT_RS18600 overlap by 4 nucleotides BPHYT_RS18600 and BPHYT_RS18605 are separated by 26 nucleotides BPHYT_RS18605 and BPHYT_RS18610 are separated by 82 nucleotides
BPHYT_RS18595: BPHYT_RS18595 - multifunctional CCA protein, at 4,182,788 to 4,184,020
_RS18595
BPHYT_RS18600: BPHYT_RS18600 - glutathione S-transferase, at 4,184,017 to 4,184,670
_RS18600
BPHYT_RS18605: BPHYT_RS18605 - NAD-dependent dehydratase, at 4,184,697 to 4,185,650
_RS18605
BPHYT_RS18610: BPHYT_RS18610 - lytic transglycosylase, at 4,185,733 to 4,187,700
_RS18610
Position (kb)
4184
4185
4186 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4184.011 kb on - strand at 4184.115 kb on - strand, within BPHYT_RS18600 at 4184.611 kb on + strand at 4184.643 kb on - strand at 4184.677 kb on + strand at 4184.678 kb on - strand at 4184.758 kb on + strand at 4184.971 kb on + strand, within BPHYT_RS18605 at 4185.019 kb on + strand, within BPHYT_RS18605 at 4185.020 kb on - strand, within BPHYT_RS18605 at 4185.020 kb on - strand, within BPHYT_RS18605 at 4185.086 kb on - strand, within BPHYT_RS18605 at 4185.265 kb on + strand, within BPHYT_RS18605 at 4185.265 kb on + strand, within BPHYT_RS18605 at 4185.266 kb on - strand, within BPHYT_RS18605 at 4185.266 kb on - strand, within BPHYT_RS18605 at 4185.305 kb on + strand, within BPHYT_RS18605 at 4185.305 kb on + strand, within BPHYT_RS18605 at 4185.305 kb on + strand, within BPHYT_RS18605 at 4185.305 kb on + strand, within BPHYT_RS18605 at 4185.305 kb on + strand at 4185.306 kb on - strand, within BPHYT_RS18605 at 4185.306 kb on - strand, within BPHYT_RS18605 at 4185.539 kb on - strand, within BPHYT_RS18605 at 4185.608 kb on + strand at 4185.608 kb on + strand at 4185.627 kb on - strand at 4185.744 kb on + strand at 4185.801 kb on - strand at 4185.830 kb on + strand at 4185.831 kb on - strand at 4185.993 kb on - strand, within BPHYT_RS18610 at 4185.993 kb on - strand, within BPHYT_RS18610 at 4186.545 kb on - strand, within BPHYT_RS18610
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO2 MOI 1 remove 4,184,011 - +1.2 4,184,115 - BPHYT_RS18600 0.15 +1.0 4,184,611 + +0.6 4,184,643 - +0.2 4,184,677 + -1.7 4,184,678 - +0.9 4,184,758 + -1.1 4,184,971 + BPHYT_RS18605 0.29 -0.5 4,185,019 + BPHYT_RS18605 0.34 -1.0 4,185,020 - BPHYT_RS18605 0.34 -0.1 4,185,020 - BPHYT_RS18605 0.34 -0.3 4,185,086 - BPHYT_RS18605 0.41 -0.7 4,185,265 + BPHYT_RS18605 0.60 +0.2 4,185,265 + BPHYT_RS18605 0.60 +0.8 4,185,266 - BPHYT_RS18605 0.60 -0.2 4,185,266 - BPHYT_RS18605 0.60 -0.1 4,185,305 + BPHYT_RS18605 0.64 +2.3 4,185,305 + BPHYT_RS18605 0.64 +0.2 4,185,305 + BPHYT_RS18605 0.64 -0.7 4,185,305 + BPHYT_RS18605 0.64 -2.5 4,185,305 + -0.0 4,185,306 - BPHYT_RS18605 0.64 -0.5 4,185,306 - BPHYT_RS18605 0.64 +1.1 4,185,539 - BPHYT_RS18605 0.88 +0.1 4,185,608 + -0.6 4,185,608 + +0.4 4,185,627 - +0.9 4,185,744 + +0.3 4,185,801 - -0.9 4,185,830 + +0.6 4,185,831 - +0.3 4,185,993 - BPHYT_RS18610 0.13 +0.6 4,185,993 - BPHYT_RS18610 0.13 -1.7 4,186,545 - BPHYT_RS18610 0.41 -2.0
Or see this region's nucleotide sequence