Experiment: 36hr PHYTO2 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS17305 and BPHYT_RS17310 are separated by 218 nucleotides BPHYT_RS17310 and BPHYT_RS17315 are separated by 189 nucleotides BPHYT_RS17315 and BPHYT_RS17320 are separated by 314 nucleotides BPHYT_RS17320 and BPHYT_RS17325 are separated by 68 nucleotides BPHYT_RS17325 and BPHYT_RS17330 are separated by 17 nucleotides
BPHYT_RS17305: BPHYT_RS17305 - amidohydrolase, at 3,922,516 to 3,923,712
_RS17305
BPHYT_RS17310: BPHYT_RS17310 - 5S ribosomal RNA, at 3,923,931 to 3,924,044
_RS17310
BPHYT_RS17315: BPHYT_RS17315 - 23S ribosomal RNA, at 3,924,234 to 3,927,136
_RS17315
BPHYT_RS17320: BPHYT_RS17320 - tRNA-Ala, at 3,927,451 to 3,927,526
_RS17320
BPHYT_RS17325: BPHYT_RS17325 - tRNA-Ile, at 3,927,595 to 3,927,671
_RS17325
BPHYT_RS17330: BPHYT_RS17330 - 16S ribosomal RNA, at 3,927,689 to 3,929,283
_RS17330
Position (kb)
3924
3925
3926
3927
3928 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3923.304 kb on + strand, within BPHYT_RS17305 at 3923.305 kb on - strand, within BPHYT_RS17305 at 3923.363 kb on - strand, within BPHYT_RS17305 at 3923.630 kb on - strand at 3923.630 kb on - strand at 3923.710 kb on + strand at 3923.710 kb on + strand at 3923.710 kb on + strand at 3923.711 kb on - strand at 3923.711 kb on - strand at 3923.759 kb on - strand at 3923.804 kb on + strand at 3923.804 kb on + strand at 3923.805 kb on - strand at 3923.805 kb on - strand at 3923.805 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO2 MOI 1 remove 3,923,304 + BPHYT_RS17305 0.66 -0.6 3,923,305 - BPHYT_RS17305 0.66 -0.2 3,923,363 - BPHYT_RS17305 0.71 -0.6 3,923,630 - +1.9 3,923,630 - +2.5 3,923,710 + -0.8 3,923,710 + -0.5 3,923,710 + -0.5 3,923,711 - +0.7 3,923,711 - -0.8 3,923,759 - -2.6 3,923,804 + +0.9 3,923,804 + +0.9 3,923,805 - -1.8 3,923,805 - +0.5 3,923,805 - -0.4
Or see this region's nucleotide sequence