Experiment: 36hr PHYTO2 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS17015 and BPHYT_RS17020 are separated by 3 nucleotides BPHYT_RS17020 and BPHYT_RS17025 are separated by 48 nucleotides BPHYT_RS17025 and BPHYT_RS17030 are separated by 73 nucleotides BPHYT_RS17030 and BPHYT_RS17035 are separated by 94 nucleotides
BPHYT_RS17015: BPHYT_RS17015 - acetyl-CoA carboxylase biotin carboxylase subunit, at 3,857,221 to 3,858,588
_RS17015
BPHYT_RS17020: BPHYT_RS17020 - ribosomal protein L11 methyltransferase, at 3,858,592 to 3,859,494
_RS17020
BPHYT_RS17025: BPHYT_RS17025 - hypothetical protein, at 3,859,543 to 3,860,943
_RS17025
BPHYT_RS17030: BPHYT_RS17030 - lipid hydroperoxide peroxidase, at 3,861,017 to 3,861,517
_RS17030
BPHYT_RS17035: BPHYT_RS17035 - sugar kinase, at 3,861,612 to 3,862,550
_RS17035
Position (kb)
3859
3860
3861 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 3859.044 kb on + strand, within BPHYT_RS17020 at 3859.212 kb on + strand, within BPHYT_RS17020 at 3859.240 kb on + strand, within BPHYT_RS17020 at 3859.241 kb on - strand, within BPHYT_RS17020 at 3859.241 kb on - strand, within BPHYT_RS17020 at 3859.241 kb on - strand, within BPHYT_RS17020 at 3859.290 kb on + strand, within BPHYT_RS17020 at 3859.293 kb on + strand, within BPHYT_RS17020 at 3859.293 kb on + strand, within BPHYT_RS17020 at 3859.293 kb on + strand, within BPHYT_RS17020 at 3859.294 kb on - strand, within BPHYT_RS17020 at 3859.294 kb on - strand, within BPHYT_RS17020 at 3859.412 kb on - strand at 3859.412 kb on - strand at 3859.437 kb on - strand at 3859.493 kb on - strand at 3859.493 kb on - strand at 3859.493 kb on - strand at 3859.504 kb on + strand at 3859.504 kb on + strand at 3859.505 kb on - strand at 3859.505 kb on - strand at 3859.542 kb on + strand at 3859.542 kb on + strand at 3859.542 kb on + strand at 3859.649 kb on + strand at 3859.733 kb on + strand, within BPHYT_RS17025 at 3860.002 kb on + strand, within BPHYT_RS17025 at 3860.003 kb on - strand, within BPHYT_RS17025 at 3860.028 kb on + strand, within BPHYT_RS17025 at 3860.128 kb on + strand, within BPHYT_RS17025 at 3860.563 kb on + strand, within BPHYT_RS17025 at 3860.564 kb on - strand, within BPHYT_RS17025 at 3860.564 kb on - strand, within BPHYT_RS17025 at 3860.587 kb on + strand, within BPHYT_RS17025 at 3860.587 kb on + strand, within BPHYT_RS17025 at 3860.818 kb on + strand at 3860.941 kb on + strand at 3861.054 kb on + strand at 3861.365 kb on + strand, within BPHYT_RS17030 at 3861.515 kb on + strand at 3861.515 kb on + strand at 3861.596 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO2 MOI 1 remove 3,859,044 + BPHYT_RS17020 0.50 -1.1 3,859,212 + BPHYT_RS17020 0.69 +0.2 3,859,240 + BPHYT_RS17020 0.72 -0.5 3,859,241 - BPHYT_RS17020 0.72 -1.2 3,859,241 - BPHYT_RS17020 0.72 -0.8 3,859,241 - BPHYT_RS17020 0.72 +0.9 3,859,290 + BPHYT_RS17020 0.77 -1.1 3,859,293 + BPHYT_RS17020 0.78 -0.3 3,859,293 + BPHYT_RS17020 0.78 -1.2 3,859,293 + BPHYT_RS17020 0.78 -1.6 3,859,294 - BPHYT_RS17020 0.78 -0.4 3,859,294 - BPHYT_RS17020 0.78 -0.3 3,859,412 - -2.1 3,859,412 - -0.8 3,859,437 - +4.7 3,859,493 - +1.1 3,859,493 - +0.4 3,859,493 - +1.6 3,859,504 + -1.5 3,859,504 + -0.1 3,859,505 - +1.2 3,859,505 - +2.5 3,859,542 + -0.4 3,859,542 + -1.6 3,859,542 + +2.5 3,859,649 + -1.1 3,859,733 + BPHYT_RS17025 0.14 +1.2 3,860,002 + BPHYT_RS17025 0.33 +0.1 3,860,003 - BPHYT_RS17025 0.33 +2.5 3,860,028 + BPHYT_RS17025 0.35 +0.1 3,860,128 + BPHYT_RS17025 0.42 -0.3 3,860,563 + BPHYT_RS17025 0.73 -0.0 3,860,564 - BPHYT_RS17025 0.73 +2.5 3,860,564 - BPHYT_RS17025 0.73 -1.9 3,860,587 + BPHYT_RS17025 0.75 -0.0 3,860,587 + BPHYT_RS17025 0.75 -0.6 3,860,818 + -0.0 3,860,941 + +1.0 3,861,054 + -0.9 3,861,365 + BPHYT_RS17030 0.69 +0.6 3,861,515 + -0.2 3,861,515 + -1.2 3,861,596 + -0.2
Or see this region's nucleotide sequence