Experiment: 36hr PHYTO2 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS16315 and BPHYT_RS16320 are separated by 244 nucleotides BPHYT_RS16320 and BPHYT_RS16325 are separated by 98 nucleotides BPHYT_RS16325 and BPHYT_RS16330 are separated by 158 nucleotides BPHYT_RS16330 and BPHYT_RS16335 are separated by 143 nucleotides
BPHYT_RS16315: BPHYT_RS16315 - peptidase M48 Ste24p, at 3,701,945 to 3,703,654
_RS16315
BPHYT_RS16320: BPHYT_RS16320 - cyclic pyranopterin monophosphate synthase accessory protein, at 3,703,899 to 3,704,375
_RS16320
BPHYT_RS16325: BPHYT_RS16325 - polymerase, at 3,704,474 to 3,706,258
_RS16325
BPHYT_RS16330: BPHYT_RS16330 - general secretion pathway protein H, at 3,706,417 to 3,707,040
_RS16330
BPHYT_RS16335: BPHYT_RS16335 - membrane protein, at 3,707,184 to 3,707,888
_RS16335
Position (kb)
3704
3705
3706
3707 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 3703.564 kb on + strand at 3703.703 kb on - strand at 3703.703 kb on - strand at 3703.727 kb on + strand at 3703.727 kb on + strand at 3703.868 kb on + strand at 3703.868 kb on + strand at 3703.868 kb on + strand at 3703.868 kb on + strand at 3703.869 kb on - strand at 3703.873 kb on + strand at 3703.874 kb on - strand at 3703.885 kb on + strand at 3703.940 kb on + strand at 3703.940 kb on + strand at 3703.940 kb on + strand at 3704.277 kb on + strand, within BPHYT_RS16320 at 3704.278 kb on - strand, within BPHYT_RS16320 at 3704.278 kb on - strand, within BPHYT_RS16320 at 3704.278 kb on - strand, within BPHYT_RS16320 at 3704.408 kb on + strand at 3704.467 kb on + strand at 3704.468 kb on - strand at 3704.593 kb on - strand at 3706.294 kb on + strand at 3706.354 kb on - strand at 3706.842 kb on - strand, within BPHYT_RS16330 at 3706.865 kb on + strand, within BPHYT_RS16330 at 3707.012 kb on + strand at 3707.022 kb on - strand at 3707.022 kb on - strand at 3707.091 kb on + strand at 3707.091 kb on + strand at 3707.092 kb on - strand at 3707.092 kb on - strand at 3707.092 kb on - strand at 3707.153 kb on - strand at 3707.153 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO2 MOI 1 remove 3,703,564 + -0.5 3,703,703 - +0.7 3,703,703 - -0.1 3,703,727 + +0.2 3,703,727 + -1.9 3,703,868 + -0.8 3,703,868 + -1.5 3,703,868 + +2.2 3,703,868 + -0.4 3,703,869 - +0.1 3,703,873 + +0.5 3,703,874 - +0.3 3,703,885 + +4.1 3,703,940 + +2.5 3,703,940 + +0.3 3,703,940 + +0.6 3,704,277 + BPHYT_RS16320 0.79 -1.1 3,704,278 - BPHYT_RS16320 0.79 -0.9 3,704,278 - BPHYT_RS16320 0.79 -1.4 3,704,278 - BPHYT_RS16320 0.79 -2.6 3,704,408 + -2.0 3,704,467 + -1.5 3,704,468 - +1.4 3,704,593 - -1.1 3,706,294 + +0.1 3,706,354 - -0.4 3,706,842 - BPHYT_RS16330 0.68 -0.9 3,706,865 + BPHYT_RS16330 0.72 -0.7 3,707,012 + +0.7 3,707,022 - -0.9 3,707,022 - -1.4 3,707,091 + +1.2 3,707,091 + -0.0 3,707,092 - -1.0 3,707,092 - -1.3 3,707,092 - +0.2 3,707,153 - -0.2 3,707,153 - +0.5
Or see this region's nucleotide sequence