Experiment: 36hr PHYTO2 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS15395 and BPHYT_RS15400 overlap by 4 nucleotides BPHYT_RS15400 and BPHYT_RS15405 are separated by 149 nucleotides BPHYT_RS15405 and BPHYT_RS15410 are separated by 106 nucleotides
BPHYT_RS15395: BPHYT_RS15395 - 6,7-dimethyl-8-ribityllumazine synthase, at 3,494,922 to 3,495,428
_RS15395
BPHYT_RS15400: BPHYT_RS15400 - N utilization substance protein B homolog, at 3,495,425 to 3,495,859
_RS15400
BPHYT_RS15405: BPHYT_RS15405 - aminotransferase, at 3,496,009 to 3,497,205
_RS15405
BPHYT_RS15410: BPHYT_RS15410 - hypothetical protein, at 3,497,312 to 3,498,493
_RS15410
Position (kb)
3496
3497
3498 Strain fitness (log2 ratio)
-1
0
1
2 at 3495.474 kb on - strand, within BPHYT_RS15400 at 3495.956 kb on + strand at 3495.956 kb on + strand at 3495.957 kb on - strand at 3495.957 kb on - strand at 3496.003 kb on - strand at 3496.008 kb on + strand at 3496.072 kb on + strand at 3496.073 kb on - strand at 3496.073 kb on - strand at 3496.134 kb on + strand, within BPHYT_RS15405 at 3496.134 kb on + strand, within BPHYT_RS15405 at 3496.135 kb on - strand, within BPHYT_RS15405 at 3496.135 kb on - strand, within BPHYT_RS15405 at 3496.219 kb on + strand, within BPHYT_RS15405 at 3496.220 kb on - strand, within BPHYT_RS15405 at 3496.270 kb on + strand, within BPHYT_RS15405 at 3496.271 kb on - strand, within BPHYT_RS15405 at 3496.282 kb on + strand, within BPHYT_RS15405 at 3496.282 kb on + strand, within BPHYT_RS15405 at 3496.282 kb on + strand, within BPHYT_RS15405 at 3496.282 kb on + strand, within BPHYT_RS15405 at 3496.282 kb on + strand, within BPHYT_RS15405 at 3496.402 kb on + strand, within BPHYT_RS15405 at 3496.403 kb on - strand, within BPHYT_RS15405 at 3496.458 kb on + strand, within BPHYT_RS15405 at 3496.626 kb on + strand, within BPHYT_RS15405 at 3496.645 kb on + strand, within BPHYT_RS15405 at 3496.645 kb on + strand, within BPHYT_RS15405 at 3496.645 kb on + strand, within BPHYT_RS15405 at 3496.646 kb on - strand, within BPHYT_RS15405 at 3496.646 kb on - strand, within BPHYT_RS15405 at 3496.729 kb on + strand, within BPHYT_RS15405 at 3496.987 kb on + strand, within BPHYT_RS15405 at 3496.987 kb on + strand, within BPHYT_RS15405 at 3496.988 kb on - strand, within BPHYT_RS15405 at 3496.988 kb on - strand, within BPHYT_RS15405 at 3497.046 kb on + strand, within BPHYT_RS15405 at 3497.122 kb on + strand at 3497.122 kb on + strand at 3497.122 kb on + strand at 3497.619 kb on + strand, within BPHYT_RS15410 at 3497.620 kb on - strand, within BPHYT_RS15410 at 3497.734 kb on - strand, within BPHYT_RS15410 at 3497.806 kb on - strand, within BPHYT_RS15410
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO2 MOI 1 remove 3,495,474 - BPHYT_RS15400 0.11 +0.9 3,495,956 + -1.3 3,495,956 + -1.1 3,495,957 - +0.5 3,495,957 - -0.1 3,496,003 - -1.5 3,496,008 + -0.5 3,496,072 + -0.8 3,496,073 - +0.6 3,496,073 - +2.5 3,496,134 + BPHYT_RS15405 0.10 +0.0 3,496,134 + BPHYT_RS15405 0.10 -0.2 3,496,135 - BPHYT_RS15405 0.11 -0.8 3,496,135 - BPHYT_RS15405 0.11 +1.4 3,496,219 + BPHYT_RS15405 0.18 -0.1 3,496,220 - BPHYT_RS15405 0.18 -0.2 3,496,270 + BPHYT_RS15405 0.22 -0.1 3,496,271 - BPHYT_RS15405 0.22 +0.2 3,496,282 + BPHYT_RS15405 0.23 +0.2 3,496,282 + BPHYT_RS15405 0.23 +0.3 3,496,282 + BPHYT_RS15405 0.23 -0.3 3,496,282 + BPHYT_RS15405 0.23 -1.6 3,496,282 + BPHYT_RS15405 0.23 -0.4 3,496,402 + BPHYT_RS15405 0.33 +0.5 3,496,403 - BPHYT_RS15405 0.33 -1.7 3,496,458 + BPHYT_RS15405 0.38 -0.8 3,496,626 + BPHYT_RS15405 0.52 -0.1 3,496,645 + BPHYT_RS15405 0.53 -0.5 3,496,645 + BPHYT_RS15405 0.53 -1.6 3,496,645 + BPHYT_RS15405 0.53 -0.7 3,496,646 - BPHYT_RS15405 0.53 +0.2 3,496,646 - BPHYT_RS15405 0.53 +0.4 3,496,729 + BPHYT_RS15405 0.60 +0.4 3,496,987 + BPHYT_RS15405 0.82 -1.1 3,496,987 + BPHYT_RS15405 0.82 -0.6 3,496,988 - BPHYT_RS15405 0.82 +0.3 3,496,988 - BPHYT_RS15405 0.82 -0.5 3,497,046 + BPHYT_RS15405 0.87 -0.5 3,497,122 + +1.1 3,497,122 + +0.5 3,497,122 + -0.1 3,497,619 + BPHYT_RS15410 0.26 -0.3 3,497,620 - BPHYT_RS15410 0.26 -1.0 3,497,734 - BPHYT_RS15410 0.36 -1.3 3,497,806 - BPHYT_RS15410 0.42 -1.7
Or see this region's nucleotide sequence