Experiment: 36hr PHYTO2 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS15045 and BPHYT_RS15050 are separated by 150 nucleotides BPHYT_RS15050 and BPHYT_RS15055 are separated by 207 nucleotides BPHYT_RS15055 and BPHYT_RS15060 are separated by 7 nucleotides
BPHYT_RS15045: BPHYT_RS15045 - peptidase C26, at 3,421,870 to 3,423,351
_RS15045
BPHYT_RS15050: BPHYT_RS15050 - potassium transporter, at 3,423,502 to 3,425,259
_RS15050
BPHYT_RS15055: BPHYT_RS15055 - hypothetical protein, at 3,425,467 to 3,426,156
_RS15055
BPHYT_RS15060: BPHYT_RS15060 - MFS transporter, at 3,426,164 to 3,427,513
_RS15060
Position (kb)
3423
3424
3425
3426 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3423.389 kb on + strand at 3423.390 kb on - strand at 3423.714 kb on + strand, within BPHYT_RS15050 at 3423.715 kb on - strand, within BPHYT_RS15050 at 3423.806 kb on + strand, within BPHYT_RS15050 at 3423.911 kb on + strand, within BPHYT_RS15050 at 3423.911 kb on + strand, within BPHYT_RS15050 at 3423.923 kb on + strand, within BPHYT_RS15050 at 3424.052 kb on + strand, within BPHYT_RS15050 at 3424.053 kb on - strand, within BPHYT_RS15050 at 3424.134 kb on - strand, within BPHYT_RS15050 at 3424.424 kb on - strand, within BPHYT_RS15050 at 3424.626 kb on - strand, within BPHYT_RS15050 at 3424.626 kb on - strand, within BPHYT_RS15050 at 3424.944 kb on - strand, within BPHYT_RS15050 at 3425.008 kb on - strand, within BPHYT_RS15050 at 3425.123 kb on + strand at 3425.263 kb on + strand at 3425.264 kb on - strand at 3425.264 kb on - strand at 3425.300 kb on + strand at 3425.346 kb on - strand at 3425.346 kb on - strand at 3425.492 kb on + strand at 3425.492 kb on + strand at 3425.492 kb on + strand at 3425.493 kb on - strand at 3425.757 kb on + strand, within BPHYT_RS15055 at 3426.124 kb on + strand at 3426.145 kb on - strand at 3426.145 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO2 MOI 1 remove 3,423,389 + -0.5 3,423,390 - -0.3 3,423,714 + BPHYT_RS15050 0.12 -0.4 3,423,715 - BPHYT_RS15050 0.12 -1.2 3,423,806 + BPHYT_RS15050 0.17 -1.3 3,423,911 + BPHYT_RS15050 0.23 -0.7 3,423,911 + BPHYT_RS15050 0.23 -0.5 3,423,923 + BPHYT_RS15050 0.24 +0.3 3,424,052 + BPHYT_RS15050 0.31 -0.5 3,424,053 - BPHYT_RS15050 0.31 +2.7 3,424,134 - BPHYT_RS15050 0.36 -0.9 3,424,424 - BPHYT_RS15050 0.52 -3.5 3,424,626 - BPHYT_RS15050 0.64 +0.0 3,424,626 - BPHYT_RS15050 0.64 -0.1 3,424,944 - BPHYT_RS15050 0.82 -0.4 3,425,008 - BPHYT_RS15050 0.86 -1.9 3,425,123 + -2.2 3,425,263 + -2.5 3,425,264 - -1.2 3,425,264 - +0.1 3,425,300 + +0.0 3,425,346 - -1.5 3,425,346 - -2.3 3,425,492 + +0.2 3,425,492 + -0.3 3,425,492 + +0.2 3,425,493 - -0.4 3,425,757 + BPHYT_RS15055 0.42 +1.8 3,426,124 + +0.3 3,426,145 - -1.7 3,426,145 - -1.0
Or see this region's nucleotide sequence