Experiment: 36hr PHYTO2 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS12845 and BPHYT_RS12850 overlap by 4 nucleotides BPHYT_RS12850 and BPHYT_RS12855 are separated by 223 nucleotides BPHYT_RS12855 and BPHYT_RS12860 are separated by 40 nucleotides
BPHYT_RS12845: BPHYT_RS12845 - ATPase, at 2,929,607 to 2,932,123
_RS12845
BPHYT_RS12850: BPHYT_RS12850 - LuxR family transcriptional regulator, at 2,932,120 to 2,932,764
_RS12850
BPHYT_RS12855: BPHYT_RS12855 - hypothetical protein, at 2,932,988 to 2,933,338
_RS12855
BPHYT_RS12860: BPHYT_RS12860 - hypothetical protein, at 2,933,379 to 2,934,320
_RS12860
Position (kb)
2932
2933
2934 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 2932.119 kb on + strand at 2932.119 kb on + strand at 2932.119 kb on + strand at 2932.120 kb on - strand at 2932.125 kb on + strand at 2932.763 kb on - strand at 2932.854 kb on - strand at 2932.892 kb on + strand at 2933.228 kb on + strand, within BPHYT_RS12855 at 2933.307 kb on - strand at 2933.308 kb on + strand at 2933.308 kb on + strand at 2933.309 kb on - strand at 2933.340 kb on - strand at 2933.389 kb on + strand at 2933.390 kb on - strand at 2933.649 kb on + strand, within BPHYT_RS12860 at 2933.649 kb on + strand, within BPHYT_RS12860 at 2933.650 kb on - strand, within BPHYT_RS12860 at 2933.650 kb on - strand, within BPHYT_RS12860 at 2933.650 kb on - strand, within BPHYT_RS12860 at 2933.650 kb on - strand, within BPHYT_RS12860 at 2933.650 kb on - strand, within BPHYT_RS12860 at 2933.650 kb on - strand, within BPHYT_RS12860 at 2933.650 kb on - strand, within BPHYT_RS12860 at 2933.774 kb on - strand, within BPHYT_RS12860 at 2933.774 kb on - strand, within BPHYT_RS12860 at 2933.774 kb on - strand, within BPHYT_RS12860 at 2933.880 kb on + strand, within BPHYT_RS12860 at 2933.880 kb on + strand, within BPHYT_RS12860 at 2933.880 kb on + strand, within BPHYT_RS12860 at 2933.895 kb on + strand, within BPHYT_RS12860 at 2933.922 kb on + strand, within BPHYT_RS12860 at 2933.923 kb on - strand, within BPHYT_RS12860 at 2934.120 kb on + strand, within BPHYT_RS12860 at 2934.120 kb on + strand, within BPHYT_RS12860 at 2934.121 kb on - strand, within BPHYT_RS12860 at 2934.121 kb on - strand, within BPHYT_RS12860 at 2934.177 kb on + strand, within BPHYT_RS12860 at 2934.177 kb on + strand, within BPHYT_RS12860
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO2 MOI 1 remove 2,932,119 + +2.4 2,932,119 + +0.5 2,932,119 + +1.3 2,932,120 - +2.2 2,932,125 + +3.1 2,932,763 - +1.3 2,932,854 - -0.0 2,932,892 + +0.2 2,933,228 + BPHYT_RS12855 0.68 -0.7 2,933,307 - +3.3 2,933,308 + +2.3 2,933,308 + +0.2 2,933,309 - +0.7 2,933,340 - +0.1 2,933,389 + -2.1 2,933,390 - +0.4 2,933,649 + BPHYT_RS12860 0.29 +0.2 2,933,649 + BPHYT_RS12860 0.29 -0.1 2,933,650 - BPHYT_RS12860 0.29 +0.4 2,933,650 - BPHYT_RS12860 0.29 +0.2 2,933,650 - BPHYT_RS12860 0.29 +0.6 2,933,650 - BPHYT_RS12860 0.29 +0.4 2,933,650 - BPHYT_RS12860 0.29 -0.1 2,933,650 - BPHYT_RS12860 0.29 +0.8 2,933,650 - BPHYT_RS12860 0.29 -1.0 2,933,774 - BPHYT_RS12860 0.42 +3.0 2,933,774 - BPHYT_RS12860 0.42 -0.3 2,933,774 - BPHYT_RS12860 0.42 +4.5 2,933,880 + BPHYT_RS12860 0.53 +0.8 2,933,880 + BPHYT_RS12860 0.53 +0.1 2,933,880 + BPHYT_RS12860 0.53 +0.2 2,933,895 + BPHYT_RS12860 0.55 -0.1 2,933,922 + BPHYT_RS12860 0.58 -1.8 2,933,923 - BPHYT_RS12860 0.58 +0.6 2,934,120 + BPHYT_RS12860 0.79 -1.8 2,934,120 + BPHYT_RS12860 0.79 -0.8 2,934,121 - BPHYT_RS12860 0.79 +0.4 2,934,121 - BPHYT_RS12860 0.79 +0.2 2,934,177 + BPHYT_RS12860 0.85 +0.7 2,934,177 + BPHYT_RS12860 0.85 -1.1
Or see this region's nucleotide sequence