Experiment: 36hr PHYTO2 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS11260 and BPHYT_RS11265 are separated by 198 nucleotides BPHYT_RS11265 and BPHYT_RS11270 are separated by 298 nucleotides BPHYT_RS11270 and BPHYT_RS11275 are separated by 45 nucleotides
BPHYT_RS11260: BPHYT_RS11260 - LysR family transcriptional regulator, at 2,558,929 to 2,559,813
_RS11260
BPHYT_RS11265: BPHYT_RS11265 - hypothetical protein, at 2,560,012 to 2,560,527
_RS11265
BPHYT_RS11270: BPHYT_RS11270 - hypothetical protein, at 2,560,826 to 2,561,263
_RS11270
BPHYT_RS11275: BPHYT_RS11275 - hypothetical protein, at 2,561,309 to 2,561,677
_RS11275
Position (kb)
2560
2561 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2559.423 kb on + strand, within BPHYT_RS11260 at 2559.509 kb on + strand, within BPHYT_RS11260 at 2559.510 kb on - strand, within BPHYT_RS11260 at 2559.618 kb on - strand, within BPHYT_RS11260 at 2559.841 kb on - strand at 2559.856 kb on - strand at 2559.856 kb on - strand at 2559.869 kb on + strand at 2559.883 kb on + strand at 2559.886 kb on + strand at 2559.886 kb on + strand at 2559.886 kb on + strand at 2559.887 kb on - strand at 2559.887 kb on - strand at 2559.889 kb on + strand at 2560.029 kb on - strand at 2560.316 kb on - strand, within BPHYT_RS11265 at 2560.526 kb on - strand at 2560.526 kb on - strand at 2560.530 kb on - strand at 2560.581 kb on + strand at 2561.033 kb on + strand, within BPHYT_RS11270 at 2561.033 kb on + strand, within BPHYT_RS11270 at 2561.034 kb on - strand, within BPHYT_RS11270 at 2561.246 kb on + strand at 2561.247 kb on - strand at 2561.370 kb on + strand, within BPHYT_RS11275 at 2561.370 kb on + strand, within BPHYT_RS11275 at 2561.371 kb on - strand, within BPHYT_RS11275 at 2561.515 kb on + strand, within BPHYT_RS11275
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO2 MOI 1 remove 2,559,423 + BPHYT_RS11260 0.56 +0.4 2,559,509 + BPHYT_RS11260 0.66 -0.4 2,559,510 - BPHYT_RS11260 0.66 -0.4 2,559,618 - BPHYT_RS11260 0.78 +0.7 2,559,841 - -0.7 2,559,856 - +0.6 2,559,856 - -0.8 2,559,869 + +1.0 2,559,883 + -0.8 2,559,886 + +1.6 2,559,886 + -1.1 2,559,886 + +0.1 2,559,887 - -0.3 2,559,887 - +0.6 2,559,889 + -2.8 2,560,029 - -0.1 2,560,316 - BPHYT_RS11265 0.59 -1.8 2,560,526 - +0.1 2,560,526 - -1.8 2,560,530 - +0.6 2,560,581 + +1.5 2,561,033 + BPHYT_RS11270 0.47 -1.3 2,561,033 + BPHYT_RS11270 0.47 -0.4 2,561,034 - BPHYT_RS11270 0.47 -0.8 2,561,246 + -1.0 2,561,247 - -2.6 2,561,370 + BPHYT_RS11275 0.17 -2.0 2,561,370 + BPHYT_RS11275 0.17 +1.8 2,561,371 - BPHYT_RS11275 0.17 +0.5 2,561,515 + BPHYT_RS11275 0.56 -0.1
Or see this region's nucleotide sequence