Experiment: 36hr PHYTO2 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS11085 and BPHYT_RS11090 are separated by 10 nucleotides BPHYT_RS11090 and BPHYT_RS11095 are separated by 35 nucleotides BPHYT_RS11095 and BPHYT_RS11100 overlap by 4 nucleotides BPHYT_RS11100 and BPHYT_RS11105 are separated by 24 nucleotides
BPHYT_RS11085: BPHYT_RS11085 - major facilitator transporter, at 2,519,848 to 2,521,245
_RS11085
BPHYT_RS11090: BPHYT_RS11090 - membrane protein, at 2,521,256 to 2,521,747
_RS11090
BPHYT_RS11095: BPHYT_RS11095 - glycosyl transferase, at 2,521,783 to 2,522,439
_RS11095
BPHYT_RS11100: BPHYT_RS11100 - methyltransferase, at 2,522,436 to 2,523,041
_RS11100
BPHYT_RS11105: BPHYT_RS11105 - acyl-CoA dehydrogenase, at 2,523,066 to 2,524,244
_RS11105
Position (kb) 2521
2522
2523 Strain fitness (log2 ratio) -2
-1
0
1
2 at 2521.727 kb on + strand at 2521.970 kb on + strand, within BPHYT_RS11095 at 2522.033 kb on + strand, within BPHYT_RS11095 at 2522.439 kb on + strand at 2522.439 kb on + strand at 2522.687 kb on - strand, within BPHYT_RS11100 at 2523.022 kb on + strand at 2523.251 kb on + strand, within BPHYT_RS11105 at 2523.311 kb on + strand, within BPHYT_RS11105
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO2 MOI 1 remove 2,521,727 + +0.2 2,521,970 + BPHYT_RS11095 0.28 -0.3 2,522,033 + BPHYT_RS11095 0.38 -0.6 2,522,439 + -2.5 2,522,439 + -1.9 2,522,687 - BPHYT_RS11100 0.41 +0.4 2,523,022 + -1.2 2,523,251 + BPHYT_RS11105 0.16 -0.5 2,523,311 + BPHYT_RS11105 0.21 +1.8
Or see this region's nucleotide sequence