Experiment: 36hr PHYTO2 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS10610 and BPHYT_RS10615 are separated by 36 nucleotides BPHYT_RS10615 and BPHYT_RS10620 are separated by 56 nucleotides BPHYT_RS10620 and BPHYT_RS10625 are separated by 119 nucleotides
BPHYT_RS10610: BPHYT_RS10610 - LysR family transcriptional regulator, at 2,418,493 to 2,419,389
_RS10610
BPHYT_RS10615: BPHYT_RS10615 - phosphonoacetaldehyde hydrolase, at 2,419,426 to 2,420,229
_RS10615
BPHYT_RS10620: BPHYT_RS10620 - 2-aminoethylphosphonate:pyruvate aminotransferase, at 2,420,286 to 2,421,518
_RS10620
BPHYT_RS10625: BPHYT_RS10625 - LysR family transcriptional regulator, at 2,421,638 to 2,422,546
_RS10625
Position (kb)
2420
2421
2422 Strain fitness (log2 ratio)
-1
0
1 at 2419.395 kb on + strand at 2419.410 kb on + strand at 2419.411 kb on - strand at 2419.467 kb on - strand at 2419.505 kb on + strand at 2419.736 kb on + strand, within BPHYT_RS10615 at 2419.746 kb on - strand, within BPHYT_RS10615 at 2419.746 kb on - strand, within BPHYT_RS10615 at 2421.053 kb on + strand, within BPHYT_RS10620 at 2421.107 kb on - strand, within BPHYT_RS10620 at 2421.129 kb on - strand, within BPHYT_RS10620 at 2421.175 kb on + strand, within BPHYT_RS10620 at 2421.247 kb on + strand, within BPHYT_RS10620 at 2421.320 kb on + strand, within BPHYT_RS10620 at 2421.561 kb on - strand at 2421.853 kb on + strand, within BPHYT_RS10625 at 2422.287 kb on - strand, within BPHYT_RS10625 at 2422.489 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO2 MOI 1 remove 2,419,395 + -0.6 2,419,410 + -0.5 2,419,411 - -0.2 2,419,467 - -0.5 2,419,505 + +0.9 2,419,736 + BPHYT_RS10615 0.39 -1.0 2,419,746 - BPHYT_RS10615 0.40 -0.4 2,419,746 - BPHYT_RS10615 0.40 +0.0 2,421,053 + BPHYT_RS10620 0.62 -1.3 2,421,107 - BPHYT_RS10620 0.67 -0.7 2,421,129 - BPHYT_RS10620 0.68 +1.6 2,421,175 + BPHYT_RS10620 0.72 -0.1 2,421,247 + BPHYT_RS10620 0.78 -1.5 2,421,320 + BPHYT_RS10620 0.84 +0.1 2,421,561 - +0.5 2,421,853 + BPHYT_RS10625 0.24 -1.4 2,422,287 - BPHYT_RS10625 0.71 +1.3 2,422,489 - -0.9
Or see this region's nucleotide sequence