Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS10615

Experiment: 36hr PHYTO2 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS10610 and BPHYT_RS10615 are separated by 36 nucleotidesBPHYT_RS10615 and BPHYT_RS10620 are separated by 56 nucleotides BPHYT_RS10610: BPHYT_RS10610 - LysR family transcriptional regulator, at 2,418,493 to 2,419,389 _RS10610 BPHYT_RS10615: BPHYT_RS10615 - phosphonoacetaldehyde hydrolase, at 2,419,426 to 2,420,229 _RS10615 BPHYT_RS10620: BPHYT_RS10620 - 2-aminoethylphosphonate:pyruvate aminotransferase, at 2,420,286 to 2,421,518 _RS10620 Position (kb) 2419 2420 2421Strain fitness (log2 ratio) -1 0 1at 2418.836 kb on - strand, within BPHYT_RS10610at 2418.936 kb on + strand, within BPHYT_RS10610at 2418.936 kb on + strand, within BPHYT_RS10610at 2418.937 kb on - strand, within BPHYT_RS10610at 2419.395 kb on + strandat 2419.410 kb on + strandat 2419.411 kb on - strandat 2419.467 kb on - strandat 2419.505 kb on + strandat 2419.736 kb on + strand, within BPHYT_RS10615at 2419.746 kb on - strand, within BPHYT_RS10615at 2419.746 kb on - strand, within BPHYT_RS10615at 2421.053 kb on + strand, within BPHYT_RS10620at 2421.107 kb on - strand, within BPHYT_RS10620at 2421.129 kb on - strand, within BPHYT_RS10620at 2421.175 kb on + strand, within BPHYT_RS10620

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Per-strain Table

Position Strand Gene LocusTag Fraction 36hr PHYTO2 MOI 1
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2,418,836 - BPHYT_RS10610 0.38 -0.2
2,418,936 + BPHYT_RS10610 0.49 +0.2
2,418,936 + BPHYT_RS10610 0.49 -0.4
2,418,937 - BPHYT_RS10610 0.49 +0.7
2,419,395 + -0.6
2,419,410 + -0.5
2,419,411 - -0.2
2,419,467 - -0.5
2,419,505 + +0.9
2,419,736 + BPHYT_RS10615 0.39 -1.0
2,419,746 - BPHYT_RS10615 0.40 -0.4
2,419,746 - BPHYT_RS10615 0.40 +0.0
2,421,053 + BPHYT_RS10620 0.62 -1.3
2,421,107 - BPHYT_RS10620 0.67 -0.7
2,421,129 - BPHYT_RS10620 0.68 +1.6
2,421,175 + BPHYT_RS10620 0.72 -0.1

Or see this region's nucleotide sequence