Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS10405

Experiment: 36hr PHYTO2 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS10395 and BPHYT_RS10400 are separated by 45 nucleotidesBPHYT_RS10400 and BPHYT_RS10405 are separated by 34 nucleotidesBPHYT_RS10405 and BPHYT_RS10410 are separated by 46 nucleotides BPHYT_RS10395: BPHYT_RS10395 - sulfonate ABC transporter, at 2,362,610 to 2,363,470 _RS10395 BPHYT_RS10400: BPHYT_RS10400 - taurine ABC transporter permease, at 2,363,516 to 2,364,388 _RS10400 BPHYT_RS10405: BPHYT_RS10405 - sulfonate ABC transporter ATP-binding protein, at 2,364,423 to 2,365,319 _RS10405 BPHYT_RS10410: BPHYT_RS10410 - monooxygenase, at 2,365,366 to 2,366,739 _RS10410 Position (kb) 2364 2365 2366Strain fitness (log2 ratio) -1 0 1 2at 2363.489 kb on + strandat 2364.066 kb on - strand, within BPHYT_RS10400at 2365.703 kb on - strandat 2365.859 kb on - strand, within BPHYT_RS10410at 2365.859 kb on - strand, within BPHYT_RS10410

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Per-strain Table

Position Strand Gene LocusTag Fraction 36hr PHYTO2 MOI 1
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2,363,489 + -0.1
2,364,066 - BPHYT_RS10400 0.63 +1.9
2,365,703 - +0.4
2,365,859 - BPHYT_RS10410 0.36 -0.5
2,365,859 - BPHYT_RS10410 0.36 -0.8

Or see this region's nucleotide sequence