Experiment: 36hr PHYTO2 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS10180 and BPHYT_RS10185 are separated by 97 nucleotides BPHYT_RS10185 and BPHYT_RS10190 are separated by 176 nucleotides BPHYT_RS10190 and BPHYT_RS10195 are separated by 76 nucleotides
BPHYT_RS10180: BPHYT_RS10180 - NADPH:quinone oxidoreductase, at 2,317,932 to 2,318,933
_RS10180
BPHYT_RS10185: BPHYT_RS10185 - MarR family transcriptional regulator, at 2,319,031 to 2,319,528
_RS10185
BPHYT_RS10190: BPHYT_RS10190 - phosphonate monoester hydrolase, at 2,319,705 to 2,321,276
_RS10190
BPHYT_RS10195: BPHYT_RS10195 - major facilitator transporter, at 2,321,353 to 2,322,690
_RS10195
Position (kb)
2319
2320
2321
2322 Strain fitness (log2 ratio)
-2
-1
0
1 at 2318.744 kb on + strand, within BPHYT_RS10180 at 2319.182 kb on + strand, within BPHYT_RS10185 at 2319.182 kb on + strand at 2319.182 kb on + strand, within BPHYT_RS10185 at 2319.428 kb on - strand, within BPHYT_RS10185 at 2319.604 kb on - strand at 2319.627 kb on + strand at 2319.627 kb on + strand at 2319.784 kb on - strand at 2319.784 kb on - strand at 2319.844 kb on - strand at 2319.844 kb on - strand at 2319.885 kb on + strand, within BPHYT_RS10190 at 2320.308 kb on + strand, within BPHYT_RS10190 at 2320.308 kb on + strand, within BPHYT_RS10190 at 2320.309 kb on - strand, within BPHYT_RS10190 at 2320.377 kb on + strand, within BPHYT_RS10190 at 2320.398 kb on + strand, within BPHYT_RS10190 at 2320.398 kb on + strand, within BPHYT_RS10190 at 2320.428 kb on + strand, within BPHYT_RS10190 at 2320.755 kb on + strand, within BPHYT_RS10190 at 2320.941 kb on + strand, within BPHYT_RS10190 at 2320.942 kb on - strand, within BPHYT_RS10190 at 2321.237 kb on + strand at 2321.437 kb on + strand at 2321.438 kb on - strand at 2321.467 kb on - strand at 2321.467 kb on - strand at 2322.023 kb on + strand, within BPHYT_RS10195 at 2322.023 kb on + strand, within BPHYT_RS10195
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO2 MOI 1 remove 2,318,744 + BPHYT_RS10180 0.81 -0.3 2,319,182 + BPHYT_RS10185 0.30 -1.6 2,319,182 + -0.4 2,319,182 + BPHYT_RS10185 0.30 +0.4 2,319,428 - BPHYT_RS10185 0.80 -1.4 2,319,604 - -0.6 2,319,627 + -0.4 2,319,627 + +1.2 2,319,784 - -1.8 2,319,784 - +0.9 2,319,844 - +1.7 2,319,844 - -0.4 2,319,885 + BPHYT_RS10190 0.11 -1.3 2,320,308 + BPHYT_RS10190 0.38 -2.0 2,320,308 + BPHYT_RS10190 0.38 +1.2 2,320,309 - BPHYT_RS10190 0.38 -1.0 2,320,377 + BPHYT_RS10190 0.43 -1.0 2,320,398 + BPHYT_RS10190 0.44 +0.2 2,320,398 + BPHYT_RS10190 0.44 -1.6 2,320,428 + BPHYT_RS10190 0.46 -0.9 2,320,755 + BPHYT_RS10190 0.67 -2.0 2,320,941 + BPHYT_RS10190 0.79 +0.5 2,320,942 - BPHYT_RS10190 0.79 +0.2 2,321,237 + +1.6 2,321,437 + +0.7 2,321,438 - -0.7 2,321,467 - +0.4 2,321,467 - +1.5 2,322,023 + BPHYT_RS10195 0.50 -0.4 2,322,023 + BPHYT_RS10195 0.50 +0.1
Or see this region's nucleotide sequence