Experiment: 36hr PHYTO2 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS07305 and BPHYT_RS07310 are separated by 8 nucleotides BPHYT_RS07310 and BPHYT_RS07315 are separated by 205 nucleotides BPHYT_RS07315 and BPHYT_RS07320 are separated by 89 nucleotides
BPHYT_RS07305: BPHYT_RS07305 - hydroxyacid dehydrogenase, at 1,658,022 to 1,658,966
_RS07305
BPHYT_RS07310: BPHYT_RS07310 - glyoxalase, at 1,658,975 to 1,659,403
_RS07310
BPHYT_RS07315: BPHYT_RS07315 - lipase, at 1,659,609 to 1,660,565
_RS07315
BPHYT_RS07320: BPHYT_RS07320 - dihydrodipicolinate synthetase, at 1,660,655 to 1,661,563
_RS07320
Position (kb) 1659
1660
1661 Strain fitness (log2 ratio) -2
-1
0
1
2 at 1658.884 kb on + strand at 1658.884 kb on + strand at 1658.885 kb on - strand at 1658.885 kb on - strand at 1658.953 kb on + strand at 1658.953 kb on + strand at 1658.954 kb on - strand at 1659.090 kb on + strand, within BPHYT_RS07310 at 1659.090 kb on + strand, within BPHYT_RS07310 at 1659.129 kb on + strand, within BPHYT_RS07310 at 1659.130 kb on - strand, within BPHYT_RS07310 at 1659.292 kb on - strand, within BPHYT_RS07310 at 1659.292 kb on - strand, within BPHYT_RS07310 at 1659.292 kb on - strand, within BPHYT_RS07310 at 1659.988 kb on - strand, within BPHYT_RS07315 at 1660.140 kb on + strand, within BPHYT_RS07315 at 1660.140 kb on + strand, within BPHYT_RS07315 at 1660.140 kb on + strand, within BPHYT_RS07315 at 1660.141 kb on - strand, within BPHYT_RS07315 at 1660.141 kb on - strand, within BPHYT_RS07315 at 1660.270 kb on - strand, within BPHYT_RS07315 at 1660.387 kb on - strand, within BPHYT_RS07315 at 1660.490 kb on + strand at 1660.490 kb on + strand at 1660.491 kb on - strand at 1660.616 kb on - strand at 1661.408 kb on - strand, within BPHYT_RS07320
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO2 MOI 1 remove 1,658,884 + -1.0 1,658,884 + -2.1 1,658,885 - -1.2 1,658,885 - +1.5 1,658,953 + -0.2 1,658,953 + +0.2 1,658,954 - -0.3 1,659,090 + BPHYT_RS07310 0.27 -1.3 1,659,090 + BPHYT_RS07310 0.27 +1.0 1,659,129 + BPHYT_RS07310 0.36 -0.4 1,659,130 - BPHYT_RS07310 0.36 +1.4 1,659,292 - BPHYT_RS07310 0.74 -0.0 1,659,292 - BPHYT_RS07310 0.74 -0.6 1,659,292 - BPHYT_RS07310 0.74 -0.5 1,659,988 - BPHYT_RS07315 0.40 -1.5 1,660,140 + BPHYT_RS07315 0.55 -0.6 1,660,140 + BPHYT_RS07315 0.55 -0.9 1,660,140 + BPHYT_RS07315 0.55 +0.2 1,660,141 - BPHYT_RS07315 0.56 +0.3 1,660,141 - BPHYT_RS07315 0.56 +2.0 1,660,270 - BPHYT_RS07315 0.69 +0.2 1,660,387 - BPHYT_RS07315 0.81 -0.4 1,660,490 + -0.8 1,660,490 + -0.9 1,660,491 - +0.8 1,660,616 - -0.5 1,661,408 - BPHYT_RS07320 0.83 -1.8
Or see this region's nucleotide sequence