Experiment: 36hr PHYTO2 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS05855 and BPHYT_RS05860 overlap by 76 nucleotides BPHYT_RS05860 and BPHYT_RS05865 are separated by 2 nucleotides BPHYT_RS05865 and BPHYT_RS05870 are separated by 138 nucleotides BPHYT_RS05870 and BPHYT_RS05875 are separated by 245 nucleotides
BPHYT_RS05855: BPHYT_RS05855 - restriction endonuclease subunit M, at 1,330,442 to 1,331,176
_RS05855
BPHYT_RS05860: BPHYT_RS05860 - ATP-binding protein, at 1,331,101 to 1,331,889
_RS05860
BPHYT_RS05865: BPHYT_RS05865 - integrase, at 1,331,892 to 1,333,418
_RS05865
BPHYT_RS05870: BPHYT_RS05870 - hypothetical protein, at 1,333,557 to 1,333,856
_RS05870
BPHYT_RS05875: BPHYT_RS05875 - acyltransferase, at 1,334,102 to 1,335,166
_RS05875
Position (kb)
1331
1332
1333
1334 Strain fitness (log2 ratio)
-1
0
1 at 1330.895 kb on - strand, within BPHYT_RS05855 at 1330.952 kb on - strand, within BPHYT_RS05855 at 1330.977 kb on - strand, within BPHYT_RS05855 at 1331.012 kb on + strand, within BPHYT_RS05855 at 1331.012 kb on + strand, within BPHYT_RS05855 at 1333.638 kb on + strand, within BPHYT_RS05870 at 1333.647 kb on + strand, within BPHYT_RS05870 at 1333.647 kb on + strand, within BPHYT_RS05870 at 1333.648 kb on - strand, within BPHYT_RS05870 at 1333.648 kb on - strand, within BPHYT_RS05870 at 1333.850 kb on - strand at 1334.145 kb on - strand at 1334.266 kb on + strand, within BPHYT_RS05875 at 1334.361 kb on - strand, within BPHYT_RS05875
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO2 MOI 1 remove 1,330,895 - BPHYT_RS05855 0.62 -0.0 1,330,952 - BPHYT_RS05855 0.69 +0.6 1,330,977 - BPHYT_RS05855 0.73 -0.2 1,331,012 + BPHYT_RS05855 0.78 +0.2 1,331,012 + BPHYT_RS05855 0.78 -0.3 1,333,638 + BPHYT_RS05870 0.27 -0.4 1,333,647 + BPHYT_RS05870 0.30 -0.8 1,333,647 + BPHYT_RS05870 0.30 -1.3 1,333,648 - BPHYT_RS05870 0.30 -1.5 1,333,648 - BPHYT_RS05870 0.30 -0.3 1,333,850 - +0.6 1,334,145 - -0.3 1,334,266 + BPHYT_RS05875 0.15 +0.2 1,334,361 - BPHYT_RS05875 0.24 -0.8
Or see this region's nucleotide sequence