Experiment: 36hr PHYTO2 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS05055 and BPHYT_RS05060 are separated by 66 nucleotides BPHYT_RS05060 and BPHYT_RS05065 are separated by 13 nucleotides BPHYT_RS05065 and BPHYT_RS05070 are separated by 180 nucleotides
BPHYT_RS05055: BPHYT_RS05055 - flagellar hook protein FliD, at 1,164,949 to 1,166,457
_RS05055
BPHYT_RS05060: BPHYT_RS05060 - membrane protein, at 1,166,524 to 1,167,144
_RS05060
BPHYT_RS05065: BPHYT_RS05065 - DNA mismatch repair protein MutS, at 1,167,158 to 1,167,916
_RS05065
BPHYT_RS05070: BPHYT_RS05070 - thioredoxin reductase, at 1,168,097 to 1,169,098
_RS05070
Position (kb) 1166
1167
1168 Strain fitness (log2 ratio) -2
-1
0
1 at 1165.599 kb on - strand, within BPHYT_RS05055 at 1165.599 kb on - strand, within BPHYT_RS05055 at 1165.897 kb on + strand, within BPHYT_RS05055 at 1165.898 kb on - strand, within BPHYT_RS05055 at 1165.898 kb on - strand, within BPHYT_RS05055 at 1165.898 kb on - strand, within BPHYT_RS05055 at 1165.936 kb on + strand, within BPHYT_RS05055 at 1166.143 kb on - strand at 1166.314 kb on + strand at 1166.398 kb on + strand at 1166.520 kb on - strand at 1166.520 kb on - strand at 1166.603 kb on + strand, within BPHYT_RS05060 at 1166.603 kb on + strand, within BPHYT_RS05060 at 1166.639 kb on + strand, within BPHYT_RS05060 at 1166.640 kb on - strand, within BPHYT_RS05060 at 1166.640 kb on - strand, within BPHYT_RS05060 at 1166.640 kb on - strand, within BPHYT_RS05060 at 1166.640 kb on - strand, within BPHYT_RS05060 at 1166.640 kb on - strand, within BPHYT_RS05060 at 1166.815 kb on + strand, within BPHYT_RS05060 at 1166.815 kb on + strand, within BPHYT_RS05060 at 1166.815 kb on + strand, within BPHYT_RS05060 at 1166.816 kb on - strand, within BPHYT_RS05060 at 1166.816 kb on - strand, within BPHYT_RS05060 at 1166.816 kb on - strand, within BPHYT_RS05060 at 1166.937 kb on - strand, within BPHYT_RS05060 at 1166.958 kb on - strand, within BPHYT_RS05060 at 1167.299 kb on + strand, within BPHYT_RS05065 at 1167.300 kb on - strand, within BPHYT_RS05065 at 1167.734 kb on + strand, within BPHYT_RS05065 at 1167.943 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO2 MOI 1 remove 1,165,599 - BPHYT_RS05055 0.43 -1.0 1,165,599 - BPHYT_RS05055 0.43 +0.4 1,165,897 + BPHYT_RS05055 0.63 +1.2 1,165,898 - BPHYT_RS05055 0.63 -1.3 1,165,898 - BPHYT_RS05055 0.63 +0.7 1,165,898 - BPHYT_RS05055 0.63 -0.2 1,165,936 + BPHYT_RS05055 0.65 -0.2 1,166,143 - +1.6 1,166,314 + -0.6 1,166,398 + -2.4 1,166,520 - -1.8 1,166,520 - -0.9 1,166,603 + BPHYT_RS05060 0.13 +1.4 1,166,603 + BPHYT_RS05060 0.13 -0.3 1,166,639 + BPHYT_RS05060 0.19 +1.4 1,166,640 - BPHYT_RS05060 0.19 +0.3 1,166,640 - BPHYT_RS05060 0.19 +1.6 1,166,640 - BPHYT_RS05060 0.19 +0.3 1,166,640 - BPHYT_RS05060 0.19 +0.4 1,166,640 - BPHYT_RS05060 0.19 +0.6 1,166,815 + BPHYT_RS05060 0.47 +0.0 1,166,815 + BPHYT_RS05060 0.47 +0.3 1,166,815 + BPHYT_RS05060 0.47 +1.6 1,166,816 - BPHYT_RS05060 0.47 +0.4 1,166,816 - BPHYT_RS05060 0.47 -1.3 1,166,816 - BPHYT_RS05060 0.47 -0.1 1,166,937 - BPHYT_RS05060 0.67 -2.0 1,166,958 - BPHYT_RS05060 0.70 -0.2 1,167,299 + BPHYT_RS05065 0.19 -1.0 1,167,300 - BPHYT_RS05065 0.19 -0.5 1,167,734 + BPHYT_RS05065 0.76 -0.9 1,167,943 - -0.2
Or see this region's nucleotide sequence