Experiment: 36hr PHYTO2 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS05020 and BPHYT_RS05025 are separated by 545 nucleotides BPHYT_RS05025 and BPHYT_RS05030 are separated by 199 nucleotides
BPHYT_RS05020: BPHYT_RS05020 - glucose-6-phosphate 1-dehydrogenase, at 1,156,416 to 1,157,879
_RS05020
BPHYT_RS05025: BPHYT_RS05025 - sugar ABC transporter substrate-binding protein, at 1,158,425 to 1,159,672
_RS05025
BPHYT_RS05030: BPHYT_RS05030 - sugar ABC transporter permease, at 1,159,872 to 1,160,810
_RS05030
Position (kb)
1158
1159
1160 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4
5 at 1157.536 kb on + strand, within BPHYT_RS05020 at 1157.696 kb on + strand, within BPHYT_RS05020 at 1157.697 kb on - strand, within BPHYT_RS05020 at 1157.697 kb on - strand, within BPHYT_RS05020 at 1157.697 kb on - strand, within BPHYT_RS05020 at 1157.965 kb on - strand at 1157.998 kb on + strand at 1158.182 kb on - strand at 1158.419 kb on + strand at 1158.420 kb on - strand at 1158.420 kb on - strand at 1158.587 kb on + strand, within BPHYT_RS05025 at 1158.797 kb on - strand at 1158.857 kb on + strand, within BPHYT_RS05025 at 1159.175 kb on - strand, within BPHYT_RS05025 at 1159.493 kb on + strand, within BPHYT_RS05025 at 1159.530 kb on - strand, within BPHYT_RS05025 at 1159.671 kb on - strand at 1159.791 kb on + strand at 1159.792 kb on - strand at 1159.803 kb on + strand at 1159.871 kb on - strand at 1159.883 kb on + strand at 1159.884 kb on - strand at 1159.884 kb on - strand at 1160.007 kb on + strand, within BPHYT_RS05030 at 1160.174 kb on + strand, within BPHYT_RS05030
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO2 MOI 1 remove 1,157,536 + BPHYT_RS05020 0.77 +4.3 1,157,696 + BPHYT_RS05020 0.87 +1.6 1,157,697 - BPHYT_RS05020 0.88 +1.0 1,157,697 - BPHYT_RS05020 0.88 +2.3 1,157,697 - BPHYT_RS05020 0.88 +0.2 1,157,965 - +0.8 1,157,998 + +1.4 1,158,182 - -1.0 1,158,419 + -0.1 1,158,420 - -0.0 1,158,420 - +0.4 1,158,587 + BPHYT_RS05025 0.13 +0.5 1,158,797 - -0.2 1,158,857 + BPHYT_RS05025 0.35 -0.7 1,159,175 - BPHYT_RS05025 0.60 +5.5 1,159,493 + BPHYT_RS05025 0.86 -3.7 1,159,530 - BPHYT_RS05025 0.89 +1.6 1,159,671 - -0.6 1,159,791 + +0.8 1,159,792 - +1.2 1,159,803 + -1.1 1,159,871 - -1.0 1,159,883 + -0.2 1,159,884 - +0.6 1,159,884 - -0.6 1,160,007 + BPHYT_RS05030 0.14 +0.3 1,160,174 + BPHYT_RS05030 0.32 +0.2
Or see this region's nucleotide sequence