Experiment: 36hr PHYTO2 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS04180 and BPHYT_RS04185 are separated by 73 nucleotides BPHYT_RS04185 and BPHYT_RS04190 are separated by 95 nucleotides
BPHYT_RS04180: BPHYT_RS04180 - sugar ABC transporter substrate-binding protein, at 958,551 to 959,798
_RS04180
BPHYT_RS04185: BPHYT_RS04185 - diguanylate cyclase, at 959,872 to 961,116
_RS04185
BPHYT_RS04190: BPHYT_RS04190 - AraC family transcriptional regulator, at 961,212 to 962,168
_RS04190
Position (kb) 959
960
961
962 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 958.916 kb on - strand, within BPHYT_RS04180 at 958.916 kb on - strand, within BPHYT_RS04180 at 958.934 kb on + strand, within BPHYT_RS04180 at 958.956 kb on - strand, within BPHYT_RS04180 at 958.956 kb on - strand, within BPHYT_RS04180 at 959.407 kb on + strand, within BPHYT_RS04180 at 959.408 kb on - strand, within BPHYT_RS04180 at 959.518 kb on - strand, within BPHYT_RS04180 at 959.518 kb on - strand, within BPHYT_RS04180 at 959.646 kb on - strand, within BPHYT_RS04180 at 959.709 kb on + strand at 959.873 kb on + strand at 959.874 kb on - strand at 959.899 kb on - strand at 960.018 kb on - strand, within BPHYT_RS04185 at 960.018 kb on - strand, within BPHYT_RS04185 at 960.018 kb on - strand, within BPHYT_RS04185 at 960.018 kb on - strand, within BPHYT_RS04185 at 960.054 kb on + strand, within BPHYT_RS04185 at 960.056 kb on + strand, within BPHYT_RS04185 at 960.056 kb on + strand, within BPHYT_RS04185 at 960.056 kb on + strand, within BPHYT_RS04185 at 960.056 kb on + strand, within BPHYT_RS04185 at 960.056 kb on + strand, within BPHYT_RS04185 at 960.056 kb on + strand, within BPHYT_RS04185 at 960.057 kb on - strand, within BPHYT_RS04185 at 960.057 kb on - strand, within BPHYT_RS04185 at 960.276 kb on + strand, within BPHYT_RS04185 at 960.277 kb on - strand, within BPHYT_RS04185 at 960.278 kb on + strand, within BPHYT_RS04185 at 960.278 kb on + strand, within BPHYT_RS04185 at 960.278 kb on + strand, within BPHYT_RS04185 at 960.278 kb on + strand, within BPHYT_RS04185 at 960.278 kb on + strand, within BPHYT_RS04185 at 960.279 kb on - strand, within BPHYT_RS04185 at 960.380 kb on - strand, within BPHYT_RS04185 at 960.438 kb on - strand, within BPHYT_RS04185 at 960.470 kb on + strand, within BPHYT_RS04185 at 960.703 kb on - strand, within BPHYT_RS04185 at 960.703 kb on - strand, within BPHYT_RS04185 at 960.703 kb on - strand, within BPHYT_RS04185 at 960.703 kb on - strand, within BPHYT_RS04185 at 960.710 kb on + strand, within BPHYT_RS04185 at 960.710 kb on + strand, within BPHYT_RS04185 at 960.710 kb on + strand, within BPHYT_RS04185 at 960.711 kb on - strand, within BPHYT_RS04185 at 960.756 kb on - strand, within BPHYT_RS04185 at 961.040 kb on + strand at 961.040 kb on + strand at 961.041 kb on - strand at 961.224 kb on + strand at 961.224 kb on + strand at 961.224 kb on + strand at 961.225 kb on - strand at 961.225 kb on - strand at 961.267 kb on - strand at 961.356 kb on + strand, within BPHYT_RS04190 at 961.380 kb on + strand, within BPHYT_RS04190 at 961.381 kb on - strand, within BPHYT_RS04190 at 961.385 kb on - strand, within BPHYT_RS04190 at 961.672 kb on + strand, within BPHYT_RS04190 at 961.766 kb on + strand, within BPHYT_RS04190 at 961.766 kb on + strand, within BPHYT_RS04190 at 961.766 kb on + strand, within BPHYT_RS04190 at 961.767 kb on - strand, within BPHYT_RS04190 at 961.905 kb on + strand, within BPHYT_RS04190 at 961.905 kb on + strand, within BPHYT_RS04190 at 961.906 kb on - strand, within BPHYT_RS04190 at 961.906 kb on - strand, within BPHYT_RS04190 at 961.907 kb on + strand, within BPHYT_RS04190
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO2 MOI 1 remove 958,916 - BPHYT_RS04180 0.29 -0.4 958,916 - BPHYT_RS04180 0.29 -0.2 958,934 + BPHYT_RS04180 0.31 +0.3 958,956 - BPHYT_RS04180 0.32 +0.4 958,956 - BPHYT_RS04180 0.32 -0.9 959,407 + BPHYT_RS04180 0.69 -0.0 959,408 - BPHYT_RS04180 0.69 -0.3 959,518 - BPHYT_RS04180 0.77 -1.4 959,518 - BPHYT_RS04180 0.77 +3.1 959,646 - BPHYT_RS04180 0.88 -2.1 959,709 + +0.2 959,873 + +0.3 959,874 - +0.4 959,899 - -1.0 960,018 - BPHYT_RS04185 0.12 -0.4 960,018 - BPHYT_RS04185 0.12 -1.6 960,018 - BPHYT_RS04185 0.12 +1.1 960,018 - BPHYT_RS04185 0.12 -1.2 960,054 + BPHYT_RS04185 0.15 -1.0 960,056 + BPHYT_RS04185 0.15 -0.4 960,056 + BPHYT_RS04185 0.15 -1.0 960,056 + BPHYT_RS04185 0.15 +0.5 960,056 + BPHYT_RS04185 0.15 -0.4 960,056 + BPHYT_RS04185 0.15 -2.5 960,056 + BPHYT_RS04185 0.15 -0.7 960,057 - BPHYT_RS04185 0.15 -0.2 960,057 - BPHYT_RS04185 0.15 -0.0 960,276 + BPHYT_RS04185 0.32 +0.3 960,277 - BPHYT_RS04185 0.33 +0.3 960,278 + BPHYT_RS04185 0.33 +0.0 960,278 + BPHYT_RS04185 0.33 -0.7 960,278 + BPHYT_RS04185 0.33 +1.1 960,278 + BPHYT_RS04185 0.33 -0.5 960,278 + BPHYT_RS04185 0.33 -0.7 960,279 - BPHYT_RS04185 0.33 -0.7 960,380 - BPHYT_RS04185 0.41 -0.1 960,438 - BPHYT_RS04185 0.45 +0.4 960,470 + BPHYT_RS04185 0.48 -0.3 960,703 - BPHYT_RS04185 0.67 +0.3 960,703 - BPHYT_RS04185 0.67 -0.7 960,703 - BPHYT_RS04185 0.67 +0.3 960,703 - BPHYT_RS04185 0.67 +0.5 960,710 + BPHYT_RS04185 0.67 -0.1 960,710 + BPHYT_RS04185 0.67 -0.7 960,710 + BPHYT_RS04185 0.67 -1.3 960,711 - BPHYT_RS04185 0.67 -1.3 960,756 - BPHYT_RS04185 0.71 +0.0 961,040 + -1.4 961,040 + -0.8 961,041 - -0.7 961,224 + +0.4 961,224 + +0.5 961,224 + -0.8 961,225 - -0.7 961,225 - +0.1 961,267 - -0.2 961,356 + BPHYT_RS04190 0.15 -1.8 961,380 + BPHYT_RS04190 0.18 -1.4 961,381 - BPHYT_RS04190 0.18 -0.7 961,385 - BPHYT_RS04190 0.18 +0.2 961,672 + BPHYT_RS04190 0.48 -0.8 961,766 + BPHYT_RS04190 0.58 -0.5 961,766 + BPHYT_RS04190 0.58 +0.1 961,766 + BPHYT_RS04190 0.58 +1.6 961,767 - BPHYT_RS04190 0.58 -0.5 961,905 + BPHYT_RS04190 0.72 +1.8 961,905 + BPHYT_RS04190 0.72 +2.0 961,906 - BPHYT_RS04190 0.73 +0.5 961,906 - BPHYT_RS04190 0.73 -0.2 961,907 + BPHYT_RS04190 0.73 +0.2
Or see this region's nucleotide sequence