Experiment: 36hr PHYTO2 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS02495 and BPHYT_RS02500 overlap by 4 nucleotides BPHYT_RS02500 and BPHYT_RS02505 are separated by 85 nucleotides BPHYT_RS02505 and BPHYT_RS02510 are separated by 404 nucleotides
BPHYT_RS02495: BPHYT_RS02495 - ABC transporter, at 565,830 to 566,768
_RS02495
BPHYT_RS02500: BPHYT_RS02500 - spermidine/putrescine ABC transporter permease, at 566,765 to 567,556
_RS02500
BPHYT_RS02505: BPHYT_RS02505 - spermidine/putrescine ABC transporter ATPase, at 567,642 to 568,712
_RS02505
BPHYT_RS02510: BPHYT_RS02510 - C4-dicarboxylate ABC transporter, at 569,117 to 570,397
_RS02510
Position (kb) 567
568
569 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 566.880 kb on - strand, within BPHYT_RS02500 at 566.880 kb on - strand, within BPHYT_RS02500 at 567.110 kb on + strand, within BPHYT_RS02500 at 567.110 kb on + strand, within BPHYT_RS02500 at 567.110 kb on + strand, within BPHYT_RS02500 at 567.111 kb on - strand, within BPHYT_RS02500 at 567.111 kb on - strand, within BPHYT_RS02500 at 567.113 kb on - strand, within BPHYT_RS02500 at 567.171 kb on + strand, within BPHYT_RS02500 at 567.179 kb on + strand, within BPHYT_RS02500 at 567.200 kb on + strand, within BPHYT_RS02500 at 567.201 kb on - strand, within BPHYT_RS02500 at 567.517 kb on - strand at 567.671 kb on + strand at 567.672 kb on - strand at 567.672 kb on - strand at 567.672 kb on - strand at 567.672 kb on - strand at 567.672 kb on - strand at 567.737 kb on + strand at 567.891 kb on + strand, within BPHYT_RS02505 at 568.148 kb on + strand, within BPHYT_RS02505 at 568.149 kb on - strand, within BPHYT_RS02505 at 568.306 kb on - strand, within BPHYT_RS02505 at 568.612 kb on + strand at 568.710 kb on + strand at 568.936 kb on + strand at 568.936 kb on + strand at 568.961 kb on + strand at 568.962 kb on - strand at 568.962 kb on - strand at 568.962 kb on - strand at 568.962 kb on - strand at 569.030 kb on - strand at 569.055 kb on - strand at 569.128 kb on + strand at 569.134 kb on + strand at 569.144 kb on + strand at 569.145 kb on - strand at 569.196 kb on - strand at 569.354 kb on + strand, within BPHYT_RS02510 at 569.354 kb on + strand, within BPHYT_RS02510 at 569.354 kb on + strand, within BPHYT_RS02510 at 569.354 kb on + strand, within BPHYT_RS02510 at 569.474 kb on + strand, within BPHYT_RS02510 at 569.474 kb on + strand, within BPHYT_RS02510
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO2 MOI 1 remove 566,880 - BPHYT_RS02500 0.15 +0.6 566,880 - BPHYT_RS02500 0.15 +0.0 567,110 + BPHYT_RS02500 0.44 -0.3 567,110 + BPHYT_RS02500 0.44 +0.1 567,110 + BPHYT_RS02500 0.44 -0.8 567,111 - BPHYT_RS02500 0.44 -0.3 567,111 - BPHYT_RS02500 0.44 +1.0 567,113 - BPHYT_RS02500 0.44 -0.0 567,171 + BPHYT_RS02500 0.51 +0.7 567,179 + BPHYT_RS02500 0.52 -1.2 567,200 + BPHYT_RS02500 0.55 -0.8 567,201 - BPHYT_RS02500 0.55 +0.4 567,517 - -0.3 567,671 + +0.2 567,672 - -0.7 567,672 - -0.5 567,672 - -0.5 567,672 - +1.3 567,672 - -2.3 567,737 + +0.4 567,891 + BPHYT_RS02505 0.23 -0.9 568,148 + BPHYT_RS02505 0.47 +0.0 568,149 - BPHYT_RS02505 0.47 +0.3 568,306 - BPHYT_RS02505 0.62 -0.4 568,612 + +0.3 568,710 + -0.0 568,936 + -0.8 568,936 + -1.6 568,961 + -2.5 568,962 - +0.1 568,962 - -1.5 568,962 - -1.4 568,962 - +0.3 569,030 - -0.4 569,055 - +0.1 569,128 + -3.1 569,134 + +0.4 569,144 + +0.2 569,145 - -0.3 569,196 - +0.1 569,354 + BPHYT_RS02510 0.19 -1.1 569,354 + BPHYT_RS02510 0.19 +0.5 569,354 + BPHYT_RS02510 0.19 -0.4 569,354 + BPHYT_RS02510 0.19 +0.3 569,474 + BPHYT_RS02510 0.28 +0.9 569,474 + BPHYT_RS02510 0.28 -1.0
Or see this region's nucleotide sequence