Experiment: 36hr PHYTO2 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS35515 and BPHYT_RS35520 are separated by 173 nucleotides BPHYT_RS35520 and BPHYT_RS35525 are separated by 186 nucleotides BPHYT_RS35525 and BPHYT_RS35530 are separated by 22 nucleotides BPHYT_RS35530 and BPHYT_RS35535 overlap by 4 nucleotides
BPHYT_RS35515: BPHYT_RS35515 - alpha/beta hydrolase, at 3,568,477 to 3,569,172
_RS35515
BPHYT_RS35520: BPHYT_RS35520 - hypothetical protein, at 3,569,346 to 3,569,978
_RS35520
BPHYT_RS35525: BPHYT_RS35525 - hypothetical protein, at 3,570,165 to 3,570,620
_RS35525
BPHYT_RS35530: BPHYT_RS35530 - arsenate reductase, at 3,570,643 to 3,571,065
_RS35530
BPHYT_RS35535: BPHYT_RS35535 - arsenic transporter, at 3,571,062 to 3,572,147
_RS35535
Position (kb)
3570
3571 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4
5 at 3569.352 kb on + strand at 3569.377 kb on + strand at 3570.006 kb on - strand at 3570.041 kb on + strand at 3570.130 kb on - strand at 3570.182 kb on - strand at 3570.351 kb on + strand, within BPHYT_RS35525 at 3570.510 kb on + strand, within BPHYT_RS35525 at 3570.510 kb on + strand, within BPHYT_RS35525 at 3570.511 kb on - strand, within BPHYT_RS35525 at 3570.649 kb on + strand at 3570.650 kb on - strand at 3570.754 kb on + strand, within BPHYT_RS35530 at 3570.810 kb on + strand, within BPHYT_RS35530 at 3570.893 kb on - strand, within BPHYT_RS35530 at 3571.049 kb on + strand at 3571.065 kb on + strand at 3571.066 kb on - strand at 3571.313 kb on - strand, within BPHYT_RS35535 at 3571.484 kb on - strand, within BPHYT_RS35535 at 3571.599 kb on + strand, within BPHYT_RS35535 at 3571.600 kb on - strand, within BPHYT_RS35535
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO2 MOI 1 remove 3,569,352 + +1.0 3,569,377 + +0.2 3,570,006 - +0.3 3,570,041 + -0.9 3,570,130 - -1.5 3,570,182 - +0.3 3,570,351 + BPHYT_RS35525 0.41 -1.0 3,570,510 + BPHYT_RS35525 0.76 -0.2 3,570,510 + BPHYT_RS35525 0.76 -1.4 3,570,511 - BPHYT_RS35525 0.76 -1.5 3,570,649 + +5.0 3,570,650 - -3.4 3,570,754 + BPHYT_RS35530 0.26 -0.2 3,570,810 + BPHYT_RS35530 0.39 -1.9 3,570,893 - BPHYT_RS35530 0.59 -0.5 3,571,049 + -0.6 3,571,065 + +2.4 3,571,066 - +0.0 3,571,313 - BPHYT_RS35535 0.23 +1.0 3,571,484 - BPHYT_RS35535 0.39 -0.2 3,571,599 + BPHYT_RS35535 0.49 +2.4 3,571,600 - BPHYT_RS35535 0.50 -0.2
Or see this region's nucleotide sequence