Experiment: 36hr PHYTO2 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS35235 and BPHYT_RS35240 are separated by 57 nucleotides BPHYT_RS35240 and BPHYT_RS35245 are separated by 128 nucleotides BPHYT_RS35245 and BPHYT_RS35250 are separated by 55 nucleotides BPHYT_RS35250 and BPHYT_RS35255 are separated by 149 nucleotides
BPHYT_RS35235: BPHYT_RS35235 - amino acid transporter, at 3,508,703 to 3,509,320
_RS35235
BPHYT_RS35240: BPHYT_RS35240 - membrane protein, at 3,509,378 to 3,509,956
_RS35240
BPHYT_RS35245: BPHYT_RS35245 - hypothetical protein, at 3,510,085 to 3,510,261
_RS35245
BPHYT_RS35250: BPHYT_RS35250 - alcohol dehydrogenase, at 3,510,317 to 3,511,282
_RS35250
BPHYT_RS35255: BPHYT_RS35255 - AraC family transcriptional regulator, at 3,511,432 to 3,512,262
_RS35255
Position (kb)
3510
3511
3512 Strain fitness (log2 ratio)
-1
0
1 at 3509.325 kb on + strand at 3509.628 kb on + strand, within BPHYT_RS35240 at 3509.628 kb on + strand, within BPHYT_RS35240 at 3509.628 kb on + strand, within BPHYT_RS35240 at 3509.628 kb on + strand, within BPHYT_RS35240 at 3509.730 kb on + strand, within BPHYT_RS35240 at 3509.784 kb on + strand, within BPHYT_RS35240 at 3509.785 kb on - strand, within BPHYT_RS35240 at 3509.944 kb on - strand at 3510.087 kb on - strand at 3510.129 kb on + strand, within BPHYT_RS35245 at 3510.129 kb on + strand, within BPHYT_RS35245 at 3510.210 kb on - strand, within BPHYT_RS35245 at 3510.270 kb on + strand at 3510.717 kb on + strand, within BPHYT_RS35250 at 3511.869 kb on - strand, within BPHYT_RS35255 at 3511.883 kb on + strand, within BPHYT_RS35255 at 3511.995 kb on + strand, within BPHYT_RS35255 at 3512.181 kb on + strand at 3512.199 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO2 MOI 1 remove 3,509,325 + -0.7 3,509,628 + BPHYT_RS35240 0.43 -0.1 3,509,628 + BPHYT_RS35240 0.43 +0.9 3,509,628 + BPHYT_RS35240 0.43 +0.1 3,509,628 + BPHYT_RS35240 0.43 -1.7 3,509,730 + BPHYT_RS35240 0.61 +0.6 3,509,784 + BPHYT_RS35240 0.70 -0.9 3,509,785 - BPHYT_RS35240 0.70 +0.1 3,509,944 - +0.6 3,510,087 - -1.5 3,510,129 + BPHYT_RS35245 0.25 -1.2 3,510,129 + BPHYT_RS35245 0.25 +0.1 3,510,210 - BPHYT_RS35245 0.71 -0.4 3,510,270 + -0.2 3,510,717 + BPHYT_RS35250 0.41 -1.3 3,511,869 - BPHYT_RS35255 0.53 +0.1 3,511,883 + BPHYT_RS35255 0.54 +0.2 3,511,995 + BPHYT_RS35255 0.68 -0.9 3,512,181 + -0.6 3,512,199 - -0.2
Or see this region's nucleotide sequence