Experiment: 36hr PHYTO2 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS34925 and BPHYT_RS34930 are separated by 441 nucleotides BPHYT_RS34930 and BPHYT_RS34935 are separated by 1 nucleotides BPHYT_RS34935 and BPHYT_RS34940 are separated by 48 nucleotides
BPHYT_RS34925: BPHYT_RS34925 - porin, at 3,437,175 to 3,438,248
_RS34925
BPHYT_RS34930: BPHYT_RS34930 - Rieske (2Fe-2S) protein, at 3,438,690 to 3,439,004
_RS34930
BPHYT_RS34935: BPHYT_RS34935 - hypothetical protein, at 3,439,006 to 3,439,317
_RS34935
BPHYT_RS34940: BPHYT_RS34940 - 3-phenylpropionate dioxygenase, at 3,439,366 to 3,440,460
_RS34940
Position (kb) 3438
3439
3440 Strain fitness (log2 ratio) -2
-1
0
1 at 3437.842 kb on + strand, within BPHYT_RS34925 at 3437.842 kb on + strand, within BPHYT_RS34925 at 3437.861 kb on - strand, within BPHYT_RS34925 at 3438.007 kb on + strand, within BPHYT_RS34925 at 3438.171 kb on + strand at 3438.171 kb on + strand at 3438.276 kb on + strand at 3438.783 kb on + strand, within BPHYT_RS34930 at 3438.958 kb on - strand, within BPHYT_RS34930 at 3439.380 kb on + strand at 3439.438 kb on + strand at 3439.907 kb on - strand, within BPHYT_RS34940 at 3439.907 kb on - strand, within BPHYT_RS34940
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO2 MOI 1 remove 3,437,842 + BPHYT_RS34925 0.62 -1.3 3,437,842 + BPHYT_RS34925 0.62 +0.2 3,437,861 - BPHYT_RS34925 0.64 -1.2 3,438,007 + BPHYT_RS34925 0.77 +0.4 3,438,171 + -2.0 3,438,171 + -1.4 3,438,276 + -2.1 3,438,783 + BPHYT_RS34930 0.30 -1.3 3,438,958 - BPHYT_RS34930 0.85 -0.9 3,439,380 + -1.8 3,439,438 + -0.4 3,439,907 - BPHYT_RS34940 0.49 -0.4 3,439,907 - BPHYT_RS34940 0.49 +0.8
Or see this region's nucleotide sequence