Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS32495

Experiment: 36hr PHYTO2 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS32490 and BPHYT_RS32495 are separated by 33 nucleotidesBPHYT_RS32495 and BPHYT_RS32500 overlap by 4 nucleotidesBPHYT_RS32500 and BPHYT_RS32505 overlap by 4 nucleotides BPHYT_RS32490: BPHYT_RS32490 - allantoate amidohydrolase, at 2,861,449 to 2,862,729 _RS32490 BPHYT_RS32495: BPHYT_RS32495 - ABC transporter, at 2,862,763 to 2,863,464 _RS32495 BPHYT_RS32500: BPHYT_RS32500 - ABC transporter, at 2,863,461 to 2,864,228 _RS32500 BPHYT_RS32505: BPHYT_RS32505 - ABC transporter permease, at 2,864,225 to 2,865,280 _RS32505 Position (kb) 2862 2863 2864Strain fitness (log2 ratio) -1 0 1at 2862.013 kb on - strand, within BPHYT_RS32490at 2862.097 kb on - strand, within BPHYT_RS32490at 2862.130 kb on - strand, within BPHYT_RS32490at 2862.836 kb on + strand, within BPHYT_RS32495at 2863.496 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 36hr PHYTO2 MOI 1
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2,862,013 - BPHYT_RS32490 0.44 +0.5
2,862,097 - BPHYT_RS32490 0.51 -1.6
2,862,130 - BPHYT_RS32490 0.53 -0.9
2,862,836 + BPHYT_RS32495 0.10 +1.5
2,863,496 - -0.1

Or see this region's nucleotide sequence