Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS32145

Experiment: 36hr PHYTO2 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS32140 and BPHYT_RS32145 overlap by 4 nucleotidesBPHYT_RS32145 and BPHYT_RS32150 overlap by 4 nucleotidesBPHYT_RS32150 and BPHYT_RS32155 are separated by 7 nucleotides BPHYT_RS32140: BPHYT_RS32140 - DNA replication protein, at 2,783,281 to 2,785,146 _RS32140 BPHYT_RS32145: BPHYT_RS32145 - type IV secretory pathway protein VirD4, at 2,785,143 to 2,786,894 _RS32145 BPHYT_RS32150: BPHYT_RS32150 - hypothetical protein, at 2,786,891 to 2,787,295 _RS32150 BPHYT_RS32155: BPHYT_RS32155 - type IV secretion system protein VirB11, at 2,787,303 to 2,788,421 _RS32155 Position (kb) 2785 2786 2787Strain fitness (log2 ratio) -2 -1 0 1 2at 2784.629 kb on + strand, within BPHYT_RS32140at 2784.630 kb on - strand, within BPHYT_RS32140at 2784.708 kb on - strand, within BPHYT_RS32140at 2785.140 kb on + strandat 2785.140 kb on + strandat 2785.140 kb on + strandat 2785.141 kb on - strandat 2785.141 kb on - strandat 2785.328 kb on - strand, within BPHYT_RS32145at 2785.408 kb on + strand, within BPHYT_RS32145at 2785.409 kb on - strand, within BPHYT_RS32145at 2785.585 kb on + strand, within BPHYT_RS32145at 2785.826 kb on - strand, within BPHYT_RS32145at 2785.859 kb on + strand, within BPHYT_RS32145at 2785.859 kb on + strand, within BPHYT_RS32145at 2785.946 kb on - strand, within BPHYT_RS32145at 2786.135 kb on - strand, within BPHYT_RS32145at 2786.414 kb on - strand, within BPHYT_RS32145at 2786.581 kb on - strand, within BPHYT_RS32145at 2786.740 kb on + strandat 2786.847 kb on + strandat 2786.946 kb on + strand, within BPHYT_RS32150at 2787.006 kb on - strand, within BPHYT_RS32150at 2787.147 kb on + strand, within BPHYT_RS32150at 2787.710 kb on - strand, within BPHYT_RS32155at 2787.816 kb on - strand, within BPHYT_RS32155at 2787.816 kb on - strand, within BPHYT_RS32155

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Per-strain Table

Position Strand Gene LocusTag Fraction 36hr PHYTO2 MOI 1
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2,784,629 + BPHYT_RS32140 0.72 -0.5
2,784,630 - BPHYT_RS32140 0.72 -0.7
2,784,708 - BPHYT_RS32140 0.76 -0.2
2,785,140 + +0.8
2,785,140 + +1.5
2,785,140 + -0.3
2,785,141 - +2.3
2,785,141 - -1.0
2,785,328 - BPHYT_RS32145 0.11 +0.7
2,785,408 + BPHYT_RS32145 0.15 -1.0
2,785,409 - BPHYT_RS32145 0.15 +0.5
2,785,585 + BPHYT_RS32145 0.25 -0.3
2,785,826 - BPHYT_RS32145 0.39 +0.2
2,785,859 + BPHYT_RS32145 0.41 +0.2
2,785,859 + BPHYT_RS32145 0.41 +1.6
2,785,946 - BPHYT_RS32145 0.46 +0.8
2,786,135 - BPHYT_RS32145 0.57 +1.4
2,786,414 - BPHYT_RS32145 0.73 +1.4
2,786,581 - BPHYT_RS32145 0.82 -1.8
2,786,740 + +0.3
2,786,847 + -1.0
2,786,946 + BPHYT_RS32150 0.14 -0.2
2,787,006 - BPHYT_RS32150 0.28 +1.9
2,787,147 + BPHYT_RS32150 0.63 -1.2
2,787,710 - BPHYT_RS32155 0.36 +0.6
2,787,816 - BPHYT_RS32155 0.46 +0.4
2,787,816 - BPHYT_RS32155 0.46 -0.2

Or see this region's nucleotide sequence