Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS30865

Experiment: 36hr PHYTO2 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS30855 and BPHYT_RS30860 are separated by 8 nucleotidesBPHYT_RS30860 and BPHYT_RS30865 overlap by 1 nucleotidesBPHYT_RS30865 and BPHYT_RS30870 are separated by 109 nucleotidesBPHYT_RS30870 and BPHYT_RS30875 are separated by 34 nucleotides BPHYT_RS30855: BPHYT_RS30855 - sugar ABC transporter substrate-binding protein, at 2,477,576 to 2,478,664 _RS30855 BPHYT_RS30860: BPHYT_RS30860 - sugar ABC transporter permease, at 2,478,673 to 2,479,662 _RS30860 BPHYT_RS30865: BPHYT_RS30865 - sugar ABC transporter ATP-binding protein, at 2,479,662 to 2,480,432 _RS30865 BPHYT_RS30870: BPHYT_RS30870 - chromate transporter, at 2,480,542 to 2,481,078 _RS30870 BPHYT_RS30875: BPHYT_RS30875 - chromate transporter, at 2,481,113 to 2,481,682 _RS30875 Position (kb) 2479 2480 2481Strain fitness (log2 ratio) -1 0 1 2at 2478.949 kb on + strand, within BPHYT_RS30860at 2479.263 kb on + strand, within BPHYT_RS30860at 2479.960 kb on - strandat 2480.164 kb on + strand, within BPHYT_RS30865at 2480.164 kb on + strand, within BPHYT_RS30865at 2480.165 kb on - strand, within BPHYT_RS30865at 2480.284 kb on - strand, within BPHYT_RS30865at 2480.696 kb on + strand, within BPHYT_RS30870at 2480.697 kb on - strand, within BPHYT_RS30870at 2481.114 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 36hr PHYTO2 MOI 1
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2,478,949 + BPHYT_RS30860 0.28 +1.0
2,479,263 + BPHYT_RS30860 0.60 +2.4
2,479,960 - +0.8
2,480,164 + BPHYT_RS30865 0.65 -0.2
2,480,164 + BPHYT_RS30865 0.65 +0.8
2,480,165 - BPHYT_RS30865 0.65 +0.4
2,480,284 - BPHYT_RS30865 0.81 -0.4
2,480,696 + BPHYT_RS30870 0.29 -0.9
2,480,697 - BPHYT_RS30870 0.29 -1.1
2,481,114 + +0.3

Or see this region's nucleotide sequence