Experiment: 36hr PHYTO2 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS29890 and BPHYT_RS29895 are separated by 96 nucleotides BPHYT_RS29895 and BPHYT_RS29900 are separated by 62 nucleotides BPHYT_RS29900 and BPHYT_RS29905 are separated by 62 nucleotides BPHYT_RS29905 and BPHYT_RS29910 are separated by 28 nucleotides
BPHYT_RS29890: BPHYT_RS29890 - LysR family transcriptional regulator, at 2,259,392 to 2,260,339
_RS29890
BPHYT_RS29895: BPHYT_RS29895 - hypothetical protein, at 2,260,436 to 2,260,741
_RS29895
BPHYT_RS29900: BPHYT_RS29900 - NADP transhydrogenase subunit beta, at 2,260,804 to 2,261,715
_RS29900
BPHYT_RS29905: BPHYT_RS29905 - 2-hydroxyhepta-2,4-diene-1,7-dioate isomerase, at 2,261,778 to 2,262,596
_RS29905
BPHYT_RS29910: BPHYT_RS29910 - formyl-CoA transferase, at 2,262,625 to 2,263,872
_RS29910
Position (kb)
2260
2261
2262 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2259.896 kb on + strand, within BPHYT_RS29890 at 2259.896 kb on + strand, within BPHYT_RS29890 at 2260.209 kb on - strand, within BPHYT_RS29890 at 2260.390 kb on + strand at 2260.740 kb on - strand at 2261.694 kb on + strand at 2261.702 kb on + strand at 2261.703 kb on - strand at 2262.278 kb on - strand, within BPHYT_RS29905 at 2262.293 kb on + strand, within BPHYT_RS29905 at 2262.518 kb on - strand at 2262.699 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO2 MOI 1 remove 2,259,896 + BPHYT_RS29890 0.53 +1.0 2,259,896 + BPHYT_RS29890 0.53 +0.4 2,260,209 - BPHYT_RS29890 0.86 -0.0 2,260,390 + -0.9 2,260,740 - -1.6 2,261,694 + +0.2 2,261,702 + -0.0 2,261,703 - +0.1 2,262,278 - BPHYT_RS29905 0.61 -1.2 2,262,293 + BPHYT_RS29905 0.63 -1.8 2,262,518 - -2.8 2,262,699 + +0.0
Or see this region's nucleotide sequence