Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS29415

Experiment: 36hr PHYTO2 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS29410 and BPHYT_RS29415 are separated by 13 nucleotidesBPHYT_RS29415 and BPHYT_RS29420 are separated by 66 nucleotidesBPHYT_RS29420 and BPHYT_RS29425 are separated by 203 nucleotides BPHYT_RS29410: BPHYT_RS29410 - HPr kinase, at 2,153,183 to 2,154,493 _RS29410 BPHYT_RS29415: BPHYT_RS29415 - 3-hydroxyisobutyrate dehydrogenase, at 2,154,507 to 2,155,397 _RS29415 BPHYT_RS29420: BPHYT_RS29420 - GntR family transcriptional regulator, at 2,155,464 to 2,156,168 _RS29420 BPHYT_RS29425: BPHYT_RS29425 - IclR family transcriptional regulator, at 2,156,372 to 2,157,196 _RS29425 Position (kb) 2154 2155 2156Strain fitness (log2 ratio) -1 0 1at 2153.842 kb on - strand, within BPHYT_RS29410at 2154.229 kb on - strand, within BPHYT_RS29410at 2154.394 kb on + strandat 2154.394 kb on + strandat 2154.484 kb on + strandat 2154.484 kb on + strandat 2154.710 kb on - strand, within BPHYT_RS29415at 2155.408 kb on + strandat 2155.409 kb on - strandat 2155.505 kb on - strandat 2155.505 kb on - strandat 2155.654 kb on + strand, within BPHYT_RS29420at 2155.688 kb on - strand, within BPHYT_RS29420at 2155.688 kb on - strand, within BPHYT_RS29420at 2156.159 kb on + strandat 2156.286 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 36hr PHYTO2 MOI 1
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2,153,842 - BPHYT_RS29410 0.50 +1.2
2,154,229 - BPHYT_RS29410 0.80 +1.1
2,154,394 + +0.5
2,154,394 + -0.3
2,154,484 + -1.1
2,154,484 + -0.8
2,154,710 - BPHYT_RS29415 0.23 -1.4
2,155,408 + +1.5
2,155,409 - -0.1
2,155,505 - -0.2
2,155,505 - -0.1
2,155,654 + BPHYT_RS29420 0.27 -1.5
2,155,688 - BPHYT_RS29420 0.32 +0.6
2,155,688 - BPHYT_RS29420 0.32 -0.7
2,156,159 + +1.0
2,156,286 + +0.1

Or see this region's nucleotide sequence