Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS29275

Experiment: 36hr PHYTO2 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS29270 and BPHYT_RS29275 are separated by 243 nucleotidesBPHYT_RS29275 and BPHYT_RS29280 are separated by 153 nucleotides BPHYT_RS29270: BPHYT_RS29270 - dehalogenase, at 2,123,176 to 2,123,844 _RS29270 BPHYT_RS29275: BPHYT_RS29275 - serine kinase, at 2,124,088 to 2,125,044 _RS29275 BPHYT_RS29280: BPHYT_RS29280 - 4-aminobutyrate aminotransferase, at 2,125,198 to 2,126,526 _RS29280 Position (kb) 2124 2125 2126Strain fitness (log2 ratio) -2 -1 0 1 2at 2123.618 kb on - strand, within BPHYT_RS29270at 2124.020 kb on + strandat 2124.209 kb on - strand, within BPHYT_RS29275at 2124.209 kb on - strand, within BPHYT_RS29275at 2124.347 kb on + strand, within BPHYT_RS29275at 2124.391 kb on + strand, within BPHYT_RS29275at 2124.398 kb on - strandat 2124.574 kb on - strand, within BPHYT_RS29275at 2124.574 kb on - strand, within BPHYT_RS29275at 2124.872 kb on - strand, within BPHYT_RS29275at 2124.998 kb on - strandat 2125.129 kb on - strandat 2125.316 kb on - strandat 2125.355 kb on - strand, within BPHYT_RS29280at 2125.394 kb on - strand, within BPHYT_RS29280at 2125.907 kb on + strand, within BPHYT_RS29280at 2125.999 kb on - strand, within BPHYT_RS29280

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Per-strain Table

Position Strand Gene LocusTag Fraction 36hr PHYTO2 MOI 1
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2,123,618 - BPHYT_RS29270 0.66 +2.2
2,124,020 + +0.8
2,124,209 - BPHYT_RS29275 0.13 -1.9
2,124,209 - BPHYT_RS29275 0.13 +0.9
2,124,347 + BPHYT_RS29275 0.27 -0.7
2,124,391 + BPHYT_RS29275 0.32 +0.1
2,124,398 - +0.8
2,124,574 - BPHYT_RS29275 0.51 -0.0
2,124,574 - BPHYT_RS29275 0.51 +0.1
2,124,872 - BPHYT_RS29275 0.82 -0.5
2,124,998 - -0.3
2,125,129 - -1.9
2,125,316 - +0.4
2,125,355 - BPHYT_RS29280 0.12 -0.0
2,125,394 - BPHYT_RS29280 0.15 -1.2
2,125,907 + BPHYT_RS29280 0.53 -1.9
2,125,999 - BPHYT_RS29280 0.60 +0.1

Or see this region's nucleotide sequence