Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS28855

Experiment: 36hr PHYTO2 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS28850 and BPHYT_RS28855 are separated by 201 nucleotidesBPHYT_RS28855 and BPHYT_RS28860 are separated by 137 nucleotidesBPHYT_RS28860 and BPHYT_RS28865 are separated by 20 nucleotides BPHYT_RS28850: BPHYT_RS28850 - hypothetical protein, at 2,023,128 to 2,024,228 _RS28850 BPHYT_RS28855: BPHYT_RS28855 - LysR family transcriptional regulator, at 2,024,430 to 2,025,335 _RS28855 BPHYT_RS28860: BPHYT_RS28860 - haloacid dehalogenase, at 2,025,473 to 2,026,144 _RS28860 BPHYT_RS28865: BPHYT_RS28865 - alanine racemase, at 2,026,165 to 2,027,304 _RS28865 Position (kb) 2024 2025 2026Strain fitness (log2 ratio) -1 0 1 2at 2023.840 kb on + strand, within BPHYT_RS28850at 2023.840 kb on + strand, within BPHYT_RS28850at 2024.230 kb on + strandat 2024.276 kb on + strandat 2024.277 kb on - strandat 2024.394 kb on + strandat 2025.406 kb on - strandat 2025.436 kb on - strandat 2026.017 kb on - strand, within BPHYT_RS28860

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Per-strain Table

Position Strand Gene LocusTag Fraction 36hr PHYTO2 MOI 1
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2,023,840 + BPHYT_RS28850 0.65 +0.4
2,023,840 + BPHYT_RS28850 0.65 -0.3
2,024,230 + +0.9
2,024,276 + +0.6
2,024,277 - +1.4
2,024,394 + +1.2
2,025,406 - +2.0
2,025,436 - -0.1
2,026,017 - BPHYT_RS28860 0.81 +1.1

Or see this region's nucleotide sequence