Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS28850

Experiment: 36hr PHYTO2 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS28840 and BPHYT_RS28845 are separated by 43 nucleotidesBPHYT_RS28845 and BPHYT_RS28850 are separated by 61 nucleotidesBPHYT_RS28850 and BPHYT_RS28855 are separated by 201 nucleotides BPHYT_RS28840: BPHYT_RS28840 - oxidoreductase, at 2,021,515 to 2,022,582 _RS28840 BPHYT_RS28845: BPHYT_RS28845 - 3-dehydroquinate dehydratase, at 2,022,626 to 2,023,066 _RS28845 BPHYT_RS28850: BPHYT_RS28850 - hypothetical protein, at 2,023,128 to 2,024,228 _RS28850 BPHYT_RS28855: BPHYT_RS28855 - LysR family transcriptional regulator, at 2,024,430 to 2,025,335 _RS28855 Position (kb) 2023 2024 2025Strain fitness (log2 ratio) -2 -1 0 1at 2022.283 kb on + strand, within BPHYT_RS28840at 2022.602 kb on - strandat 2022.693 kb on - strand, within BPHYT_RS28845at 2022.869 kb on + strand, within BPHYT_RS28845at 2022.875 kb on + strand, within BPHYT_RS28845at 2022.929 kb on - strand, within BPHYT_RS28845at 2023.840 kb on + strand, within BPHYT_RS28850at 2023.840 kb on + strand, within BPHYT_RS28850at 2024.230 kb on + strandat 2024.276 kb on + strandat 2024.277 kb on - strandat 2024.394 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 36hr PHYTO2 MOI 1
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2,022,283 + BPHYT_RS28840 0.72 -1.5
2,022,602 - +0.1
2,022,693 - BPHYT_RS28845 0.15 -0.2
2,022,869 + BPHYT_RS28845 0.55 -2.7
2,022,875 + BPHYT_RS28845 0.56 -1.2
2,022,929 - BPHYT_RS28845 0.69 -0.6
2,023,840 + BPHYT_RS28850 0.65 +0.4
2,023,840 + BPHYT_RS28850 0.65 -0.3
2,024,230 + +0.9
2,024,276 + +0.6
2,024,277 - +1.4
2,024,394 + +1.2

Or see this region's nucleotide sequence