Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS28480

Experiment: 36hr PHYTO2 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS28475 and BPHYT_RS28480 are separated by 195 nucleotidesBPHYT_RS28480 and BPHYT_RS28485 are separated by 151 nucleotides BPHYT_RS28475: BPHYT_RS28475 - porin, at 1,942,358 to 1,943,524 _RS28475 BPHYT_RS28480: BPHYT_RS28480 - hydroxyglutarate oxidase, at 1,943,720 to 1,944,916 _RS28480 BPHYT_RS28485: BPHYT_RS28485 - GntR family transcriptional regulator, at 1,945,068 to 1,945,769 _RS28485 Position (kb) 1943 1944 1945Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2 3at 1942.762 kb on - strand, within BPHYT_RS28475at 1942.956 kb on + strand, within BPHYT_RS28475at 1942.957 kb on - strand, within BPHYT_RS28475at 1942.999 kb on + strand, within BPHYT_RS28475at 1943.000 kb on - strand, within BPHYT_RS28475at 1943.029 kb on - strand, within BPHYT_RS28475at 1943.029 kb on - strand, within BPHYT_RS28475at 1943.151 kb on + strand, within BPHYT_RS28475at 1943.152 kb on - strand, within BPHYT_RS28475at 1943.214 kb on - strand, within BPHYT_RS28475at 1943.217 kb on + strand, within BPHYT_RS28475at 1943.218 kb on - strand, within BPHYT_RS28475at 1943.408 kb on + strandat 1943.409 kb on - strandat 1943.563 kb on + strandat 1943.564 kb on - strandat 1944.465 kb on - strand, within BPHYT_RS28480at 1944.598 kb on - strand, within BPHYT_RS28480at 1945.065 kb on - strandat 1945.084 kb on - strandat 1945.401 kb on - strand, within BPHYT_RS28485at 1945.805 kb on + strandat 1945.813 kb on + strandat 1945.865 kb on - strandat 1945.865 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 36hr PHYTO2 MOI 1
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1,942,762 - BPHYT_RS28475 0.35 -0.9
1,942,956 + BPHYT_RS28475 0.51 -0.1
1,942,957 - BPHYT_RS28475 0.51 +0.7
1,942,999 + BPHYT_RS28475 0.55 +0.8
1,943,000 - BPHYT_RS28475 0.55 +3.4
1,943,029 - BPHYT_RS28475 0.57 +0.2
1,943,029 - BPHYT_RS28475 0.57 -1.4
1,943,151 + BPHYT_RS28475 0.68 +0.0
1,943,152 - BPHYT_RS28475 0.68 +1.2
1,943,214 - BPHYT_RS28475 0.73 -0.7
1,943,217 + BPHYT_RS28475 0.74 +0.2
1,943,218 - BPHYT_RS28475 0.74 +1.0
1,943,408 + +2.4
1,943,409 - -0.2
1,943,563 + +0.6
1,943,564 - -1.2
1,944,465 - BPHYT_RS28480 0.62 -4.4
1,944,598 - BPHYT_RS28480 0.73 +0.0
1,945,065 - -2.1
1,945,084 - -1.3
1,945,401 - BPHYT_RS28485 0.47 -0.9
1,945,805 + -1.0
1,945,813 + -1.7
1,945,865 - -0.5
1,945,865 - -1.3

Or see this region's nucleotide sequence