Experiment: 36hr PHYTO2 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS28275 and BPHYT_RS28280 are separated by 191 nucleotides BPHYT_RS28280 and BPHYT_RS28285 are separated by 123 nucleotides BPHYT_RS28285 and BPHYT_RS28290 are separated by 382 nucleotides BPHYT_RS28290 and BPHYT_RS28295 are separated by 59 nucleotides
BPHYT_RS28275: BPHYT_RS28275 - glycerol acyltransferase, at 1,900,169 to 1,900,879
_RS28275
BPHYT_RS28280: BPHYT_RS28280 - hypothetical protein, at 1,901,071 to 1,901,385
_RS28280
BPHYT_RS28285: BPHYT_RS28285 - MFS transporter, at 1,901,509 to 1,902,756
_RS28285
BPHYT_RS28290: BPHYT_RS28290 - hypothetical protein, at 1,903,139 to 1,903,411
_RS28290
BPHYT_RS28295: BPHYT_RS28295 - hypothetical protein, at 1,903,471 to 1,903,788
_RS28295
Position (kb)
1901
1902
1903 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1900.850 kb on - strand at 1900.950 kb on + strand at 1900.951 kb on - strand at 1900.951 kb on - strand at 1901.305 kb on + strand, within BPHYT_RS28280 at 1901.305 kb on + strand, within BPHYT_RS28280 at 1902.026 kb on + strand, within BPHYT_RS28285 at 1902.756 kb on + strand at 1902.756 kb on + strand at 1902.757 kb on - strand at 1902.757 kb on - strand at 1902.759 kb on + strand at 1902.774 kb on + strand at 1902.774 kb on + strand at 1902.778 kb on - strand at 1902.778 kb on - strand at 1902.932 kb on + strand at 1902.932 kb on + strand at 1902.991 kb on - strand at 1903.145 kb on - strand at 1903.145 kb on - strand at 1903.205 kb on + strand, within BPHYT_RS28290 at 1903.206 kb on - strand, within BPHYT_RS28290 at 1903.484 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO2 MOI 1 remove 1,900,850 - -0.1 1,900,950 + -1.7 1,900,951 - -1.5 1,900,951 - +1.4 1,901,305 + BPHYT_RS28280 0.74 +2.7 1,901,305 + BPHYT_RS28280 0.74 -0.1 1,902,026 + BPHYT_RS28285 0.41 -1.8 1,902,756 + +2.1 1,902,756 + -1.3 1,902,757 - +0.3 1,902,757 - +2.4 1,902,759 + -0.5 1,902,774 + -0.3 1,902,774 + -1.0 1,902,778 - -1.6 1,902,778 - -1.5 1,902,932 + -2.5 1,902,932 + +0.2 1,902,991 - -2.2 1,903,145 - +1.2 1,903,145 - +1.2 1,903,205 + BPHYT_RS28290 0.24 -1.3 1,903,206 - BPHYT_RS28290 0.25 +0.4 1,903,484 + +1.7
Or see this region's nucleotide sequence