Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS27195

Experiment: 36hr PHYTO2 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS27190 and BPHYT_RS27195 are separated by 39 nucleotidesBPHYT_RS27195 and BPHYT_RS27200 are separated by 68 nucleotides BPHYT_RS27190: BPHYT_RS27190 - sugar ABC transporter permease, at 1,677,881 to 1,678,912 _RS27190 BPHYT_RS27195: BPHYT_RS27195 - LacI family transcriptional regulator, at 1,678,952 to 1,679,899 _RS27195 BPHYT_RS27200: BPHYT_RS27200 - pyridoxal 4-dehydrogenase, at 1,679,968 to 1,680,984 _RS27200 Position (kb) 1678 1679 1680Strain fitness (log2 ratio) -2 -1 0 1 2at 1678.603 kb on + strand, within BPHYT_RS27190at 1678.694 kb on + strand, within BPHYT_RS27190at 1678.695 kb on - strand, within BPHYT_RS27190at 1678.910 kb on + strandat 1679.067 kb on - strand, within BPHYT_RS27195at 1679.278 kb on + strand, within BPHYT_RS27195at 1679.484 kb on - strand, within BPHYT_RS27195at 1679.772 kb on - strand, within BPHYT_RS27195at 1680.055 kb on + strandat 1680.142 kb on + strand, within BPHYT_RS27200at 1680.210 kb on - strand, within BPHYT_RS27200at 1680.311 kb on - strand, within BPHYT_RS27200at 1680.312 kb on + strand, within BPHYT_RS27200at 1680.386 kb on - strand, within BPHYT_RS27200at 1680.592 kb on + strand, within BPHYT_RS27200at 1680.845 kb on + strand, within BPHYT_RS27200

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Per-strain Table

Position Strand Gene LocusTag Fraction 36hr PHYTO2 MOI 1
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1,678,603 + BPHYT_RS27190 0.70 -1.6
1,678,694 + BPHYT_RS27190 0.79 -0.3
1,678,695 - BPHYT_RS27190 0.79 +0.1
1,678,910 + +0.8
1,679,067 - BPHYT_RS27195 0.12 +1.0
1,679,278 + BPHYT_RS27195 0.34 +2.4
1,679,484 - BPHYT_RS27195 0.56 +1.4
1,679,772 - BPHYT_RS27195 0.86 -2.0
1,680,055 + -0.8
1,680,142 + BPHYT_RS27200 0.17 -0.1
1,680,210 - BPHYT_RS27200 0.24 -1.6
1,680,311 - BPHYT_RS27200 0.34 -1.3
1,680,312 + BPHYT_RS27200 0.34 -0.1
1,680,386 - BPHYT_RS27200 0.41 +0.8
1,680,592 + BPHYT_RS27200 0.61 -2.0
1,680,845 + BPHYT_RS27200 0.86 +0.8

Or see this region's nucleotide sequence