Experiment: 36hr PHYTO2 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS26735 and BPHYT_RS26740 are separated by 28 nucleotides BPHYT_RS26740 and BPHYT_RS26745 are separated by 57 nucleotides BPHYT_RS26745 and BPHYT_RS26750 are separated by 4 nucleotides BPHYT_RS26750 and BPHYT_RS26755 are separated by 53 nucleotides
BPHYT_RS26735: BPHYT_RS26735 - FAD-dependent oxidoreductase, at 1,568,340 to 1,570,091
_RS26735
BPHYT_RS26740: BPHYT_RS26740 - hypothetical protein, at 1,570,120 to 1,570,311
_RS26740
BPHYT_RS26745: BPHYT_RS26745 - 2-dehydropantoate 2-reductase, at 1,570,369 to 1,571,352
_RS26745
BPHYT_RS26750: BPHYT_RS26750 - aldolase, at 1,571,357 to 1,572,148
_RS26750
BPHYT_RS26755: BPHYT_RS26755 - aromatic amino acid aminotransferase, at 1,572,202 to 1,573,401
_RS26755
Position (kb)
1570
1571
1572 Strain fitness (log2 ratio)
-2
-1
0
1 at 1569.447 kb on - strand, within BPHYT_RS26735 at 1569.451 kb on - strand, within BPHYT_RS26735 at 1569.451 kb on - strand, within BPHYT_RS26735 at 1569.451 kb on - strand, within BPHYT_RS26735 at 1569.879 kb on - strand, within BPHYT_RS26735 at 1570.093 kb on - strand at 1570.098 kb on + strand at 1570.201 kb on + strand, within BPHYT_RS26740 at 1570.370 kb on + strand at 1571.080 kb on + strand, within BPHYT_RS26745 at 1572.005 kb on - strand, within BPHYT_RS26750 at 1572.015 kb on + strand, within BPHYT_RS26750 at 1572.243 kb on - strand at 1572.243 kb on - strand at 1572.243 kb on - strand at 1572.300 kb on - strand at 1572.300 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO2 MOI 1 remove 1,569,447 - BPHYT_RS26735 0.63 -0.0 1,569,451 - BPHYT_RS26735 0.63 -0.5 1,569,451 - BPHYT_RS26735 0.63 -0.7 1,569,451 - BPHYT_RS26735 0.63 -1.5 1,569,879 - BPHYT_RS26735 0.88 +0.1 1,570,093 - -2.2 1,570,098 + +0.2 1,570,201 + BPHYT_RS26740 0.42 +0.2 1,570,370 + -0.5 1,571,080 + BPHYT_RS26745 0.72 +0.5 1,572,005 - BPHYT_RS26750 0.82 -2.4 1,572,015 + BPHYT_RS26750 0.83 -1.2 1,572,243 - -2.0 1,572,243 - +0.2 1,572,243 - +0.1 1,572,300 - -1.4 1,572,300 - -0.6
Or see this region's nucleotide sequence