Experiment: 36hr PHYTO2 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS26725 and BPHYT_RS26730 are separated by 136 nucleotides BPHYT_RS26730 and BPHYT_RS26735 are separated by 162 nucleotides
BPHYT_RS26725: BPHYT_RS26725 - hypothetical protein, at 1,564,799 to 1,566,088
_RS26725
BPHYT_RS26730: BPHYT_RS26730 - membrane protein, at 1,566,225 to 1,568,177
_RS26730
BPHYT_RS26735: BPHYT_RS26735 - FAD-dependent oxidoreductase, at 1,568,340 to 1,570,091
_RS26735
Position (kb)
1566
1567
1568
1569 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 1565.235 kb on + strand, within BPHYT_RS26725 at 1565.236 kb on - strand, within BPHYT_RS26725 at 1565.236 kb on - strand, within BPHYT_RS26725 at 1565.236 kb on - strand, within BPHYT_RS26725 at 1565.542 kb on - strand, within BPHYT_RS26725 at 1565.911 kb on + strand, within BPHYT_RS26725 at 1565.990 kb on + strand at 1565.990 kb on + strand at 1566.118 kb on - strand at 1566.187 kb on - strand at 1566.187 kb on - strand at 1566.339 kb on + strand at 1566.830 kb on + strand, within BPHYT_RS26730 at 1566.943 kb on - strand, within BPHYT_RS26730 at 1567.068 kb on + strand, within BPHYT_RS26730 at 1567.068 kb on + strand, within BPHYT_RS26730 at 1567.148 kb on + strand, within BPHYT_RS26730 at 1567.210 kb on - strand, within BPHYT_RS26730 at 1567.244 kb on - strand, within BPHYT_RS26730 at 1567.966 kb on - strand, within BPHYT_RS26730 at 1568.193 kb on + strand at 1568.382 kb on + strand at 1568.382 kb on + strand at 1568.734 kb on - strand, within BPHYT_RS26735 at 1568.994 kb on + strand, within BPHYT_RS26735 at 1568.995 kb on - strand, within BPHYT_RS26735 at 1569.061 kb on - strand, within BPHYT_RS26735 at 1569.136 kb on - strand, within BPHYT_RS26735
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO2 MOI 1 remove 1,565,235 + BPHYT_RS26725 0.34 +0.5 1,565,236 - BPHYT_RS26725 0.34 +0.3 1,565,236 - BPHYT_RS26725 0.34 -2.1 1,565,236 - BPHYT_RS26725 0.34 -0.0 1,565,542 - BPHYT_RS26725 0.58 -0.1 1,565,911 + BPHYT_RS26725 0.86 +2.2 1,565,990 + +2.4 1,565,990 + +1.6 1,566,118 - +0.9 1,566,187 - -1.1 1,566,187 - +1.4 1,566,339 + -1.0 1,566,830 + BPHYT_RS26730 0.31 -1.9 1,566,943 - BPHYT_RS26730 0.37 +0.4 1,567,068 + BPHYT_RS26730 0.43 +2.4 1,567,068 + BPHYT_RS26730 0.43 -0.2 1,567,148 + BPHYT_RS26730 0.47 -0.3 1,567,210 - BPHYT_RS26730 0.50 +1.4 1,567,244 - BPHYT_RS26730 0.52 -0.5 1,567,966 - BPHYT_RS26730 0.89 -1.5 1,568,193 + +0.0 1,568,382 + +4.7 1,568,382 + +0.5 1,568,734 - BPHYT_RS26735 0.22 +0.8 1,568,994 + BPHYT_RS26735 0.37 +0.7 1,568,995 - BPHYT_RS26735 0.37 -0.0 1,569,061 - BPHYT_RS26735 0.41 -0.1 1,569,136 - BPHYT_RS26735 0.45 -0.1
Or see this region's nucleotide sequence