Experiment: 36hr PHYTO2 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS26585 and BPHYT_RS26590 are separated by 92 nucleotides BPHYT_RS26590 and BPHYT_RS26595 are separated by 94 nucleotides BPHYT_RS26595 and BPHYT_RS26600 are separated by 118 nucleotides
BPHYT_RS26585: BPHYT_RS26585 - trehalase, at 1,532,993 to 1,534,666
_RS26585
BPHYT_RS26590: BPHYT_RS26590 - DNA-3-methyladenine glycosylase, at 1,534,759 to 1,535,379
_RS26590
BPHYT_RS26595: BPHYT_RS26595 - carbonic anhydrase, at 1,535,474 to 1,536,211
_RS26595
BPHYT_RS26600: BPHYT_RS26600 - carbonic anhydrase, at 1,536,330 to 1,536,965
_RS26600
Position (kb) 1535
1536
1537 Strain fitness (log2 ratio) -2
-1
0
1
2 at 1534.514 kb on + strand at 1534.514 kb on + strand at 1534.938 kb on + strand, within BPHYT_RS26590 at 1534.957 kb on + strand, within BPHYT_RS26590 at 1534.957 kb on + strand, within BPHYT_RS26590 at 1534.958 kb on - strand, within BPHYT_RS26590 at 1535.091 kb on + strand, within BPHYT_RS26590 at 1535.092 kb on - strand, within BPHYT_RS26590 at 1535.285 kb on - strand, within BPHYT_RS26590 at 1535.285 kb on - strand, within BPHYT_RS26590 at 1535.508 kb on + strand at 1535.547 kb on + strand at 1535.824 kb on - strand, within BPHYT_RS26595 at 1535.997 kb on + strand, within BPHYT_RS26595 at 1536.017 kb on - strand, within BPHYT_RS26595 at 1536.224 kb on - strand at 1536.771 kb on - strand, within BPHYT_RS26600 at 1537.013 kb on - strand at 1537.015 kb on + strand at 1537.208 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO2 MOI 1 remove 1,534,514 + -0.6 1,534,514 + -0.4 1,534,938 + BPHYT_RS26590 0.29 -2.1 1,534,957 + BPHYT_RS26590 0.32 -0.5 1,534,957 + BPHYT_RS26590 0.32 +1.8 1,534,958 - BPHYT_RS26590 0.32 +1.3 1,535,091 + BPHYT_RS26590 0.53 -0.3 1,535,092 - BPHYT_RS26590 0.54 +1.2 1,535,285 - BPHYT_RS26590 0.85 +1.2 1,535,285 - BPHYT_RS26590 0.85 +0.1 1,535,508 + +0.0 1,535,547 + -2.1 1,535,824 - BPHYT_RS26595 0.47 +0.7 1,535,997 + BPHYT_RS26595 0.71 +1.3 1,536,017 - BPHYT_RS26595 0.74 -2.2 1,536,224 - +0.4 1,536,771 - BPHYT_RS26600 0.69 -0.4 1,537,013 - +0.7 1,537,015 + +0.2 1,537,208 - -0.5
Or see this region's nucleotide sequence