Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS25590

Experiment: 36hr PHYTO2 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS25585 and BPHYT_RS25590 are separated by 623 nucleotidesBPHYT_RS25590 and BPHYT_RS25595 are separated by 276 nucleotides BPHYT_RS25585: BPHYT_RS25585 - membrane protein, at 1,311,577 to 1,311,888 _RS25585 BPHYT_RS25590: BPHYT_RS25590 - FAD-dependent pyridine nucleotide-disulfide oxidoreductase, at 1,312,512 to 1,314,602 _RS25590 BPHYT_RS25595: BPHYT_RS25595 - ATPase AAA, at 1,314,879 to 1,316,528 _RS25595 Position (kb) 1312 1313 1314 1315Strain fitness (log2 ratio) -3 -2 -1 0 1at 1311.801 kb on + strand, within BPHYT_RS25585at 1311.955 kb on - strandat 1312.571 kb on + strandat 1312.572 kb on - strandat 1313.362 kb on + strand, within BPHYT_RS25590at 1313.895 kb on + strand, within BPHYT_RS25590at 1313.896 kb on - strand, within BPHYT_RS25590at 1314.095 kb on + strand, within BPHYT_RS25590

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Per-strain Table

Position Strand Gene LocusTag Fraction 36hr PHYTO2 MOI 1
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1,311,801 + BPHYT_RS25585 0.72 -0.3
1,311,955 - -1.1
1,312,571 + -2.8
1,312,572 - -1.2
1,313,362 + BPHYT_RS25590 0.41 -0.3
1,313,895 + BPHYT_RS25590 0.66 -2.2
1,313,896 - BPHYT_RS25590 0.66 -1.9
1,314,095 + BPHYT_RS25590 0.76 -1.5

Or see this region's nucleotide sequence