Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS25220

Experiment: 36hr PHYTO2 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS25215 and BPHYT_RS25220 are separated by 33 nucleotidesBPHYT_RS25220 and BPHYT_RS25225 are separated by 136 nucleotides BPHYT_RS25215: BPHYT_RS25215 - (Fe-S)-binding protein, at 1,230,792 to 1,232,105 _RS25215 BPHYT_RS25220: BPHYT_RS25220 - ferredoxin oxidoreductase, at 1,232,139 to 1,233,386 _RS25220 BPHYT_RS25225: BPHYT_RS25225 - glycine/betaine ABC transporter substrate-binding protein, at 1,233,523 to 1,234,380 _RS25225 Position (kb) 1232 1233 1234Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 1232.412 kb on + strand, within BPHYT_RS25220at 1232.666 kb on + strand, within BPHYT_RS25220at 1232.895 kb on - strand, within BPHYT_RS25220at 1233.234 kb on - strand, within BPHYT_RS25220at 1233.380 kb on + strandat 1234.136 kb on - strand, within BPHYT_RS25225

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Per-strain Table

Position Strand Gene LocusTag Fraction 36hr PHYTO2 MOI 1
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1,232,412 + BPHYT_RS25220 0.22 -0.0
1,232,666 + BPHYT_RS25220 0.42 -1.9
1,232,895 - BPHYT_RS25220 0.61 -0.2
1,233,234 - BPHYT_RS25220 0.88 -1.5
1,233,380 + -2.8
1,234,136 - BPHYT_RS25225 0.71 +2.5

Or see this region's nucleotide sequence