Experiment: 36hr PHYTO2 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS23850 and BPHYT_RS23855 are separated by 387 nucleotides BPHYT_RS23855 and BPHYT_RS23860 are separated by 31 nucleotides BPHYT_RS23860 and BPHYT_RS23865 are separated by 156 nucleotides
BPHYT_RS23850: BPHYT_RS23850 - ser/threonine protein phosphatase, at 917,858 to 919,021
_RS23850
BPHYT_RS23855: BPHYT_RS23855 - hypothetical protein, at 919,409 to 919,738
_RS23855
BPHYT_RS23860: BPHYT_RS23860 - malonic semialdehyde reductase, at 919,770 to 920,354
_RS23860
BPHYT_RS23865: BPHYT_RS23865 - 2OG-Fe(II) oxygenase, at 920,511 to 921,122
_RS23865
Position (kb) 919
920
921 Strain fitness (log2 ratio) -1
0
1
2
3 at 918.925 kb on + strand at 918.926 kb on - strand at 918.963 kb on + strand at 918.963 kb on + strand at 918.963 kb on + strand at 918.964 kb on - strand at 919.021 kb on + strand at 919.163 kb on - strand at 919.245 kb on + strand at 919.295 kb on + strand at 919.306 kb on - strand at 919.318 kb on + strand at 919.318 kb on + strand at 919.408 kb on + strand at 919.408 kb on + strand at 919.409 kb on - strand at 919.409 kb on - strand at 919.583 kb on + strand, within BPHYT_RS23855 at 919.584 kb on - strand, within BPHYT_RS23855 at 919.584 kb on - strand, within BPHYT_RS23855 at 919.719 kb on - strand at 920.241 kb on + strand, within BPHYT_RS23860 at 920.241 kb on + strand, within BPHYT_RS23860 at 920.454 kb on + strand at 920.455 kb on - strand at 920.718 kb on + strand, within BPHYT_RS23865 at 920.719 kb on - strand, within BPHYT_RS23865 at 920.719 kb on - strand, within BPHYT_RS23865 at 920.725 kb on - strand, within BPHYT_RS23865 at 920.816 kb on + strand, within BPHYT_RS23865 at 921.190 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO2 MOI 1 remove 918,925 + +1.1 918,926 - +0.8 918,963 + +1.4 918,963 + -1.2 918,963 + +0.9 918,964 - -0.7 919,021 + -1.6 919,163 - +0.2 919,245 + -0.1 919,295 + +0.6 919,306 - -0.1 919,318 + -1.6 919,318 + -0.7 919,408 + -0.0 919,408 + +0.1 919,409 - -0.4 919,409 - +0.2 919,583 + BPHYT_RS23855 0.53 +3.3 919,584 - BPHYT_RS23855 0.53 +0.5 919,584 - BPHYT_RS23855 0.53 +0.2 919,719 - -0.1 920,241 + BPHYT_RS23860 0.81 -0.9 920,241 + BPHYT_RS23860 0.81 -0.8 920,454 + +0.4 920,455 - -0.7 920,718 + BPHYT_RS23865 0.34 -0.7 920,719 - BPHYT_RS23865 0.34 -1.0 920,719 - BPHYT_RS23865 0.34 -1.5 920,725 - BPHYT_RS23865 0.35 +0.0 920,816 + BPHYT_RS23865 0.50 -0.9 921,190 - +0.5
Or see this region's nucleotide sequence