Experiment: 36hr PHYTO2 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS23845 and BPHYT_RS23850 are separated by 40 nucleotides BPHYT_RS23850 and BPHYT_RS23855 are separated by 387 nucleotides BPHYT_RS23855 and BPHYT_RS23860 are separated by 31 nucleotides
BPHYT_RS23845: BPHYT_RS23845 - hypothetical protein, at 917,293 to 917,817
_RS23845
BPHYT_RS23850: BPHYT_RS23850 - ser/threonine protein phosphatase, at 917,858 to 919,021
_RS23850
BPHYT_RS23855: BPHYT_RS23855 - hypothetical protein, at 919,409 to 919,738
_RS23855
BPHYT_RS23860: BPHYT_RS23860 - malonic semialdehyde reductase, at 919,770 to 920,354
_RS23860
Position (kb) 917
918
919
920 Strain fitness (log2 ratio) -1
0
1
2
3 at 916.945 kb on - strand at 916.945 kb on - strand at 916.945 kb on - strand at 917.860 kb on - strand at 917.955 kb on + strand at 917.956 kb on - strand at 917.956 kb on - strand at 918.135 kb on - strand, within BPHYT_RS23850 at 918.328 kb on - strand, within BPHYT_RS23850 at 918.331 kb on - strand, within BPHYT_RS23850 at 918.394 kb on + strand, within BPHYT_RS23850 at 918.395 kb on - strand, within BPHYT_RS23850 at 918.925 kb on + strand at 918.926 kb on - strand at 918.963 kb on + strand at 918.963 kb on + strand at 918.963 kb on + strand at 918.964 kb on - strand at 919.021 kb on + strand at 919.163 kb on - strand at 919.245 kb on + strand at 919.295 kb on + strand at 919.306 kb on - strand at 919.318 kb on + strand at 919.318 kb on + strand at 919.408 kb on + strand at 919.408 kb on + strand at 919.409 kb on - strand at 919.409 kb on - strand at 919.583 kb on + strand, within BPHYT_RS23855 at 919.584 kb on - strand, within BPHYT_RS23855 at 919.584 kb on - strand, within BPHYT_RS23855 at 919.719 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO2 MOI 1 remove 916,945 - -1.2 916,945 - +0.2 916,945 - +0.6 917,860 - -1.5 917,955 + -0.4 917,956 - +0.6 917,956 - -0.1 918,135 - BPHYT_RS23850 0.24 +0.1 918,328 - BPHYT_RS23850 0.40 +0.5 918,331 - BPHYT_RS23850 0.41 +0.9 918,394 + BPHYT_RS23850 0.46 -0.9 918,395 - BPHYT_RS23850 0.46 +0.3 918,925 + +1.1 918,926 - +0.8 918,963 + +1.4 918,963 + -1.2 918,963 + +0.9 918,964 - -0.7 919,021 + -1.6 919,163 - +0.2 919,245 + -0.1 919,295 + +0.6 919,306 - -0.1 919,318 + -1.6 919,318 + -0.7 919,408 + -0.0 919,408 + +0.1 919,409 - -0.4 919,409 - +0.2 919,583 + BPHYT_RS23855 0.53 +3.3 919,584 - BPHYT_RS23855 0.53 +0.5 919,584 - BPHYT_RS23855 0.53 +0.2 919,719 - -0.1
Or see this region's nucleotide sequence