Experiment: 36hr PHYTO2 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS23730 and BPHYT_RS23735 are separated by 133 nucleotides BPHYT_RS23735 and BPHYT_RS23740 are separated by 185 nucleotides
BPHYT_RS23730: BPHYT_RS23730 - multidrug DMT transporter permease, at 891,286 to 892,158
_RS23730
BPHYT_RS23735: BPHYT_RS23735 - ABC transporter, at 892,292 to 893,851
_RS23735
BPHYT_RS23740: BPHYT_RS23740 - AraC family transcriptional regulator, at 894,037 to 894,792
_RS23740
Position (kb) 892
893
894 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 891.371 kb on + strand at 891.372 kb on - strand at 891.932 kb on + strand, within BPHYT_RS23730 at 891.932 kb on + strand, within BPHYT_RS23730 at 891.959 kb on - strand, within BPHYT_RS23730 at 892.421 kb on - strand at 892.483 kb on + strand, within BPHYT_RS23735 at 892.484 kb on - strand, within BPHYT_RS23735 at 892.484 kb on - strand, within BPHYT_RS23735 at 892.565 kb on + strand, within BPHYT_RS23735 at 892.579 kb on + strand, within BPHYT_RS23735 at 892.579 kb on + strand, within BPHYT_RS23735 at 892.579 kb on + strand, within BPHYT_RS23735 at 892.580 kb on - strand, within BPHYT_RS23735 at 892.588 kb on + strand, within BPHYT_RS23735 at 892.589 kb on - strand, within BPHYT_RS23735 at 892.851 kb on + strand, within BPHYT_RS23735 at 893.146 kb on - strand, within BPHYT_RS23735 at 893.722 kb on + strand at 893.829 kb on - strand at 893.934 kb on + strand at 893.934 kb on + strand at 893.934 kb on + strand at 893.934 kb on + strand at 893.935 kb on - strand at 893.969 kb on + strand at 893.970 kb on - strand at 893.973 kb on - strand at 893.973 kb on - strand at 893.973 kb on - strand at 894.035 kb on - strand at 894.035 kb on - strand at 894.035 kb on - strand at 894.061 kb on + strand at 894.061 kb on + strand at 894.062 kb on - strand at 894.062 kb on - strand at 894.062 kb on - strand at 894.238 kb on - strand, within BPHYT_RS23740 at 894.388 kb on + strand, within BPHYT_RS23740 at 894.389 kb on - strand, within BPHYT_RS23740 at 894.473 kb on + strand, within BPHYT_RS23740 at 894.474 kb on - strand, within BPHYT_RS23740 at 894.474 kb on - strand, within BPHYT_RS23740 at 894.474 kb on - strand, within BPHYT_RS23740 at 894.583 kb on + strand, within BPHYT_RS23740 at 894.835 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO2 MOI 1 remove 891,371 + +0.4 891,372 - -0.2 891,932 + BPHYT_RS23730 0.74 +0.5 891,932 + BPHYT_RS23730 0.74 -2.2 891,959 - BPHYT_RS23730 0.77 -0.6 892,421 - -1.0 892,483 + BPHYT_RS23735 0.12 +1.0 892,484 - BPHYT_RS23735 0.12 -1.0 892,484 - BPHYT_RS23735 0.12 -0.5 892,565 + BPHYT_RS23735 0.17 -2.2 892,579 + BPHYT_RS23735 0.18 -0.7 892,579 + BPHYT_RS23735 0.18 -1.0 892,579 + BPHYT_RS23735 0.18 -0.2 892,580 - BPHYT_RS23735 0.18 -0.0 892,588 + BPHYT_RS23735 0.19 -3.1 892,589 - BPHYT_RS23735 0.19 -1.2 892,851 + BPHYT_RS23735 0.36 -1.7 893,146 - BPHYT_RS23735 0.55 -0.1 893,722 + -0.5 893,829 - -0.5 893,934 + -0.4 893,934 + +0.3 893,934 + -1.6 893,934 + +0.3 893,935 - +1.5 893,969 + -1.8 893,970 - -1.3 893,973 - +0.7 893,973 - +0.2 893,973 - -1.1 894,035 - +1.9 894,035 - -1.6 894,035 - -0.5 894,061 + -1.5 894,061 + -0.9 894,062 - -1.5 894,062 - -2.0 894,062 - -3.2 894,238 - BPHYT_RS23740 0.27 -2.9 894,388 + BPHYT_RS23740 0.46 -0.3 894,389 - BPHYT_RS23740 0.47 +1.3 894,473 + BPHYT_RS23740 0.58 -0.6 894,474 - BPHYT_RS23740 0.58 -2.1 894,474 - BPHYT_RS23740 0.58 -3.2 894,474 - BPHYT_RS23740 0.58 -1.2 894,583 + BPHYT_RS23740 0.72 -3.3 894,835 - +0.6
Or see this region's nucleotide sequence