Experiment: 36hr PHYTO2 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS23705 and BPHYT_RS23710 are separated by 113 nucleotides BPHYT_RS23710 and BPHYT_RS23715 are separated by 443 nucleotides BPHYT_RS23715 and BPHYT_RS23720 are separated by 91 nucleotides
BPHYT_RS23705: BPHYT_RS23705 - hypothetical protein, at 886,929 to 887,582
_RS23705
BPHYT_RS23710: BPHYT_RS23710 - hypothetical protein, at 887,696 to 888,046
_RS23710
BPHYT_RS23715: BPHYT_RS23715 - hypothetical protein, at 888,490 to 888,756
_RS23715
BPHYT_RS23720: BPHYT_RS23720 - nH(3)-dependent NAD(+) synthetase, at 888,848 to 889,708
_RS23720
Position (kb) 887
888
889 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 886.824 kb on - strand at 886.824 kb on - strand at 886.824 kb on - strand at 886.824 kb on - strand at 886.841 kb on - strand at 886.854 kb on + strand at 886.854 kb on + strand at 886.855 kb on - strand at 886.858 kb on + strand at 886.858 kb on + strand at 886.858 kb on + strand at 886.859 kb on - strand at 886.859 kb on - strand at 886.878 kb on + strand at 886.882 kb on + strand at 886.899 kb on + strand at 886.899 kb on + strand at 886.900 kb on - strand at 886.900 kb on - strand at 886.988 kb on + strand at 886.988 kb on + strand at 886.988 kb on + strand at 886.988 kb on + strand at 886.989 kb on - strand at 886.989 kb on - strand at 886.989 kb on - strand at 886.993 kb on - strand at 887.096 kb on - strand, within BPHYT_RS23705 at 887.096 kb on - strand, within BPHYT_RS23705 at 887.096 kb on - strand, within BPHYT_RS23705 at 887.628 kb on - strand at 887.628 kb on - strand at 887.846 kb on + strand, within BPHYT_RS23710 at 888.202 kb on + strand at 888.211 kb on - strand at 888.324 kb on - strand at 888.324 kb on - strand at 889.005 kb on + strand, within BPHYT_RS23720
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO2 MOI 1 remove 886,824 - +0.6 886,824 - -0.5 886,824 - -0.4 886,824 - +0.6 886,841 - +0.3 886,854 + +1.6 886,854 + -0.4 886,855 - -1.2 886,858 + +0.6 886,858 + -1.1 886,858 + -1.2 886,859 - -0.3 886,859 - +0.8 886,878 + -0.5 886,882 + -0.8 886,899 + +1.8 886,899 + +0.6 886,900 - +0.5 886,900 - -0.7 886,988 + -0.3 886,988 + -0.2 886,988 + -0.2 886,988 + +0.9 886,989 - +0.9 886,989 - +2.3 886,989 - +0.5 886,993 - -0.6 887,096 - BPHYT_RS23705 0.26 +0.3 887,096 - BPHYT_RS23705 0.26 -0.5 887,096 - BPHYT_RS23705 0.26 -0.4 887,628 - -3.1 887,628 - +0.2 887,846 + BPHYT_RS23710 0.43 -0.3 888,202 + -0.6 888,211 - +0.8 888,324 - -1.2 888,324 - -1.0 889,005 + BPHYT_RS23720 0.18 -1.3
Or see this region's nucleotide sequence