Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS22920

Experiment: 36hr PHYTO2 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS22915 and BPHYT_RS22920 are separated by 54 nucleotidesBPHYT_RS22920 and BPHYT_RS22925 are separated by 27 nucleotides BPHYT_RS22915: BPHYT_RS22915 - pilus assembly protein CpaE, at 699,718 to 700,920 _RS22915 BPHYT_RS22920: BPHYT_RS22920 - pilus assembly protein CpaF, at 700,975 to 702,357 _RS22920 BPHYT_RS22925: BPHYT_RS22925 - pilus assembly protein TadB, at 702,385 to 703,362 _RS22925 Position (kb) 700 701 702 703Strain fitness (log2 ratio) -1 0 1at 700.180 kb on + strand, within BPHYT_RS22915at 700.353 kb on - strand, within BPHYT_RS22915at 701.357 kb on + strand, within BPHYT_RS22920at 701.357 kb on + strandat 701.358 kb on - strand, within BPHYT_RS22920at 701.358 kb on - strand, within BPHYT_RS22920at 701.358 kb on - strand, within BPHYT_RS22920at 701.360 kb on - strand, within BPHYT_RS22920at 702.251 kb on - strandat 702.688 kb on + strand, within BPHYT_RS22925at 702.689 kb on - strand, within BPHYT_RS22925at 702.689 kb on - strand, within BPHYT_RS22925at 702.930 kb on - strand, within BPHYT_RS22925

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Per-strain Table

Position Strand Gene LocusTag Fraction 36hr PHYTO2 MOI 1
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700,180 + BPHYT_RS22915 0.38 +0.8
700,353 - BPHYT_RS22915 0.53 -0.4
701,357 + BPHYT_RS22920 0.28 +0.0
701,357 + +0.4
701,358 - BPHYT_RS22920 0.28 +0.2
701,358 - BPHYT_RS22920 0.28 +0.0
701,358 - BPHYT_RS22920 0.28 -0.7
701,360 - BPHYT_RS22920 0.28 +0.1
702,251 - -0.9
702,688 + BPHYT_RS22925 0.31 +1.4
702,689 - BPHYT_RS22925 0.31 -0.4
702,689 - BPHYT_RS22925 0.31 -1.0
702,930 - BPHYT_RS22925 0.56 -0.5

Or see this region's nucleotide sequence