Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS22525

Experiment: 36hr PHYTO2 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS22520 and BPHYT_RS22525 are separated by 375 nucleotidesBPHYT_RS22525 and BPHYT_RS22530 are separated by 43 nucleotides BPHYT_RS22520: BPHYT_RS22520 - RND transporter, at 613,083 to 614,684 _RS22520 BPHYT_RS22525: BPHYT_RS22525 - dioxygenase, at 615,060 to 615,899 _RS22525 BPHYT_RS22530: BPHYT_RS22530 - N5,N10-methylene tetrahydromethanopterin reductase, at 615,943 to 617,349 _RS22530 Position (kb) 615 616Strain fitness (log2 ratio) -2 -1 0 1 2at 614.445 kb on + strand, within BPHYT_RS22520at 614.467 kb on - strand, within BPHYT_RS22520at 614.467 kb on - strand, within BPHYT_RS22520at 614.790 kb on + strandat 614.814 kb on - strandat 615.293 kb on + strand, within BPHYT_RS22525at 615.337 kb on - strand, within BPHYT_RS22525at 615.348 kb on - strand, within BPHYT_RS22525at 615.384 kb on + strand, within BPHYT_RS22525at 615.432 kb on + strand, within BPHYT_RS22525at 616.042 kb on + strandat 616.043 kb on - strandat 616.043 kb on - strandat 616.282 kb on + strand, within BPHYT_RS22530

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Per-strain Table

Position Strand Gene LocusTag Fraction 36hr PHYTO2 MOI 1
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614,445 + BPHYT_RS22520 0.85 +0.0
614,467 - BPHYT_RS22520 0.86 -1.2
614,467 - BPHYT_RS22520 0.86 +1.1
614,790 + -0.5
614,814 - +0.6
615,293 + BPHYT_RS22525 0.28 +0.3
615,337 - BPHYT_RS22525 0.33 +0.4
615,348 - BPHYT_RS22525 0.34 +0.1
615,384 + BPHYT_RS22525 0.39 -1.5
615,432 + BPHYT_RS22525 0.44 +0.6
616,042 + +2.1
616,043 - -2.2
616,043 - +0.1
616,282 + BPHYT_RS22530 0.24 -1.7

Or see this region's nucleotide sequence