Experiment: 36hr PHYTO2 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS21960 and BPHYT_RS21965 are separated by 15 nucleotides BPHYT_RS21965 and BPHYT_RS21970 overlap by 4 nucleotides BPHYT_RS21970 and BPHYT_RS21975 are separated by 219 nucleotides
BPHYT_RS21960: BPHYT_RS21960 - nucleoside transporter, at 483,861 to 485,138
_RS21960
BPHYT_RS21965: BPHYT_RS21965 - cytidine deaminase, at 485,154 to 485,543
_RS21965
BPHYT_RS21970: BPHYT_RS21970 - nucleoside hydrolase, at 485,540 to 486,505
_RS21970
BPHYT_RS21975: BPHYT_RS21975 - 2-dehydropantoate 2-reductase, at 486,725 to 487,660
_RS21975
Position (kb) 485
486
487 Strain fitness (log2 ratio) -2
-1
0
1
2 at 484.593 kb on - strand, within BPHYT_RS21960 at 484.866 kb on + strand, within BPHYT_RS21960 at 485.015 kb on + strand at 485.535 kb on + strand at 485.536 kb on - strand at 485.540 kb on - strand at 485.822 kb on - strand, within BPHYT_RS21970 at 486.266 kb on + strand, within BPHYT_RS21970 at 486.267 kb on - strand, within BPHYT_RS21970 at 486.342 kb on + strand, within BPHYT_RS21970 at 486.636 kb on + strand at 486.736 kb on - strand at 486.738 kb on + strand at 487.046 kb on - strand, within BPHYT_RS21975 at 487.132 kb on + strand, within BPHYT_RS21975
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO2 MOI 1 remove 484,593 - BPHYT_RS21960 0.57 +0.7 484,866 + BPHYT_RS21960 0.79 +0.9 485,015 + -0.8 485,535 + -0.6 485,536 - +0.3 485,540 - +0.9 485,822 - BPHYT_RS21970 0.29 -1.5 486,266 + BPHYT_RS21970 0.75 -2.3 486,267 - BPHYT_RS21970 0.75 -0.1 486,342 + BPHYT_RS21970 0.83 -1.0 486,636 + +1.8 486,736 - +0.6 486,738 + -0.3 487,046 - BPHYT_RS21975 0.34 -1.1 487,132 + BPHYT_RS21975 0.43 -0.1
Or see this region's nucleotide sequence