Experiment: 36hr PHYTO2 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS21045 and BPHYT_RS21050 are separated by 523 nucleotides BPHYT_RS21050 and BPHYT_RS21055 are separated by 2 nucleotides
BPHYT_RS21045: BPHYT_RS21045 - bis(5'-nucleosyl)-tetraphosphatase, at 281,719 to 282,801
_RS21045
BPHYT_RS21050: BPHYT_RS21050 - aldehyde-activating protein, at 283,325 to 283,900
_RS21050
BPHYT_RS21055: BPHYT_RS21055 - S-formylglutathione hydrolase, at 283,903 to 284,733
_RS21055
Position (kb) 283
284 Strain fitness (log2 ratio) -1
0
1
2
3 at 282.618 kb on - strand, within BPHYT_RS21045 at 282.638 kb on + strand, within BPHYT_RS21045 at 282.683 kb on - strand, within BPHYT_RS21045 at 282.818 kb on - strand at 282.921 kb on + strand at 282.970 kb on + strand at 282.979 kb on + strand at 282.979 kb on + strand at 282.979 kb on + strand at 283.080 kb on + strand at 283.297 kb on + strand at 283.301 kb on - strand at 283.304 kb on - strand at 283.398 kb on + strand, within BPHYT_RS21050 at 283.398 kb on + strand, within BPHYT_RS21050 at 283.474 kb on + strand, within BPHYT_RS21050 at 283.632 kb on - strand, within BPHYT_RS21050 at 283.831 kb on - strand, within BPHYT_RS21050 at 284.007 kb on + strand, within BPHYT_RS21055 at 284.131 kb on - strand, within BPHYT_RS21055 at 284.264 kb on - strand, within BPHYT_RS21055 at 284.497 kb on + strand, within BPHYT_RS21055 at 284.668 kb on - strand at 284.668 kb on - strand at 284.675 kb on - strand at 284.810 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO2 MOI 1 remove 282,618 - BPHYT_RS21045 0.83 -0.4 282,638 + BPHYT_RS21045 0.85 +0.1 282,683 - BPHYT_RS21045 0.89 +0.3 282,818 - +0.1 282,921 + -0.6 282,970 + -0.4 282,979 + +0.9 282,979 + +0.5 282,979 + -1.1 283,080 + -0.1 283,297 + +0.9 283,301 - +1.4 283,304 - -0.2 283,398 + BPHYT_RS21050 0.13 -0.8 283,398 + BPHYT_RS21050 0.13 -0.7 283,474 + BPHYT_RS21050 0.26 -0.4 283,632 - BPHYT_RS21050 0.53 +1.1 283,831 - BPHYT_RS21050 0.88 +0.5 284,007 + BPHYT_RS21055 0.13 -1.6 284,131 - BPHYT_RS21055 0.27 +2.8 284,264 - BPHYT_RS21055 0.43 -1.0 284,497 + BPHYT_RS21055 0.71 +0.7 284,668 - +0.0 284,668 - -0.4 284,675 - -1.7 284,810 - -0.3
Or see this region's nucleotide sequence